Minnesota/8 July 2008

From 2008.igem.org

(Difference between revisions)
Line 11: Line 11:
|-
|-
|2. '''Restart/Redo PCR''': Problem with original PCR was an error in the calculation of DNA concentration of product. 07-07-2008 we added only 1.0uL of PCR to digest reactions meaning only 3ng of DNA was in the solution. That was not a substantial amount of DNA to be seen in a electrophoretic gel. FIX - recalculated DNA concentration and is 3ng/1uL instead of 300ng/uL. Redid PCR2 with 100.0ng DNA instead of 1ng of DNA by adding 10.0uL of DNA. Will run gel with new PCR undigested (original PCR was digested).   
|2. '''Restart/Redo PCR''': Problem with original PCR was an error in the calculation of DNA concentration of product. 07-07-2008 we added only 1.0uL of PCR to digest reactions meaning only 3ng of DNA was in the solution. That was not a substantial amount of DNA to be seen in a electrophoretic gel. FIX - recalculated DNA concentration and is 3ng/1uL instead of 300ng/uL. Redid PCR2 with 100.0ng DNA instead of 1ng of DNA by adding 10.0uL of DNA. Will run gel with new PCR undigested (original PCR was digested).   
-
 
+
|-
 +
|3. '''PCR''': New PCR completed successfully. The RBS has been incorporated into the lambda cI gene, and the resulting DNA has a concentration of 23 ng/ul. Analysis by agarose gel electrophoresis confirmed that the DNA fragment is of the appropriate size.
 +
|-
 +
|Image:7.8.08gel.jpg
|}
|}

Revision as of 21:43, 8 July 2008

Back to Notebook Home
Go to Previous Day (July 7)Go to Next Day (July 9)
1. Simulations: Finish typing into designgui the simulations/rxn network. NOT USING SYNBIOSS.
2. Restart/Redo PCR: Problem with original PCR was an error in the calculation of DNA concentration of product. 07-07-2008 we added only 1.0uL of PCR to digest reactions meaning only 3ng of DNA was in the solution. That was not a substantial amount of DNA to be seen in a electrophoretic gel. FIX - recalculated DNA concentration and is 3ng/1uL instead of 300ng/uL. Redid PCR2 with 100.0ng DNA instead of 1ng of DNA by adding 10.0uL of DNA. Will run gel with new PCR undigested (original PCR was digested).
3. PCR: New PCR completed successfully. The RBS has been incorporated into the lambda cI gene, and the resulting DNA has a concentration of 23 ng/ul. Analysis by agarose gel electrophoresis confirmed that the DNA fragment is of the appropriate size.
Image:7.8.08gel.jpg