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Team:Hawaii/Notebook/2008-07- 8
From 2008.igem.org
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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Completed oligonucleotide experiment=== :<strong> Grace</strong> :* Ran RE digested ligations on 4% gel. Too low DNA c...) |
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:* Extracted BBa_E0240 and BBa_I14032 using commercial grade DH5α super competent cells. | :* Extracted BBa_E0240 and BBa_I14032 using commercial grade DH5α super competent cells. | ||
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| + | [[Image:C0012_plasmid_prep.JPG|thumb|right|The two expected bands corresponding to the vector and the BioBrick insert are clearly visible.]] | ||
| + | ===Restriction Digest of C0012 plasmid prep=== | ||
| + | :<strong> Grace</strong> | ||
= Discussion = | = Discussion = | ||
Revision as of 06:16, 9 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Completed oligonucleotide experiment
- Grace
- Ran RE digested ligations on 4% gel. Too low DNA concentration so no ligated products were seen.
- Redid ligation and RE digest. Loaded 20 μl in 4% gel.
- RE digested C0012.
- Gel extraction of C0012 vector and ligated products.
- Ligated C0012 vector with pnir, slr2016, and pilA.
Biobrick extraction
- Krystle
- Extracted BBa_E0240 and BBa_I14032 using commercial grade DH5α super competent cells.
Restriction Digest of C0012 plasmid prep
- Grace
Discussion
Quote of the Day
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
