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Minnesota/9 July 2008
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|1. '''Single Digests''': Performed today. Incubated digested samples for 2hrs @ 37C. Refer to table below. | |1. '''Single Digests''': Performed today. Incubated digested samples for 2hrs @ 37C. Refer to table below. | ||
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Revision as of 16:06, 9 July 2008
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| 1. Single Digests: Performed today. Incubated digested samples for 2hrs @ 37C. Refer to table below. |
| Gene | 10x Buffer | BSA Protein | H20 | DNA | RE |
|---|---|---|---|---|---|
| Promoter | 5.0uL | 0.5uL | 40.4uL | 2.1uL | 2.0uL, SPE |
| LAMBDAcI | 5.0uL | 0.5uL | 29.5uL | 15.0uL | 1.0uL, XBA 1 |
| LAMBDAcI | 5.0uL | 0.5uL | 29.5uL | 15.0uL | 1.0uL, SPE |
| Terminator | 5.0uL | 0.5uL | 38.0uL | 4.5uL | 2.0uL, XBA 1 |
| NOTE: RE = Restriction Enzyme |
| 2. Ligation: Refer to table below. |
| Genes | 10x Buffer | H20 | Base Vector | Insert | DNA Ligase |
|---|---|---|---|---|---|
| LAMBDAcI/Terminator | 2.0uL | 10.7uL | 2.0uL (Term.) | 4.3uL (LAMBDAcI) | 1.0uL |
| Promoter/LAMBDAcI | 2.0uL | 10.7uL | 2.0uL (Pro.) | 4.3uL (LAMBDAcI) | 1.0uL |
