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User:University of Washington/9 July 2008
From 2008.igem.org
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- Plated GFP generator (BBa_E0240: received from BioCircuit Lab) on LB Agar + Amp. | - Plated GFP generator (BBa_E0240: received from BioCircuit Lab) on LB Agar + Amp. | ||
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| + | == BioBrick Promoter Measurements == | ||
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| + | - Overnight promoter-construct cultures were diluted 1:100, then incubated in a rotator for 3 hours. | ||
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| + | - Fluorescence and OD600 measurements were taken every five minutes for eight hours for the four promoter-construct cultures, an empty culture of TOP10 cells, M9 media, and M9+Kan. | ||
Revision as of 18:50, 11 July 2008
RP4 Conjugation
·Success!!! Bacteria-Bacteria conjugation works
·Received lambda red primers for RP4 manipulation
LuxR from AraC and TetR
- Took AraC plasmid after thermo cycling out from the machine, stored in the fridge.
- Plated GFP generator (BBa_E0240: received from BioCircuit Lab) on LB Agar + Amp.
BioBrick Promoter Measurements
- Overnight promoter-construct cultures were diluted 1:100, then incubated in a rotator for 3 hours.
- Fluorescence and OD600 measurements were taken every five minutes for eight hours for the four promoter-construct cultures, an empty culture of TOP10 cells, M9 media, and M9+Kan.
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