Team:KULeuven/Tools/PCR

From 2008.igem.org

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{| cellspacing="1" width=100%
{| cellspacing="1" width=100%
|style="background:#003E81; color:white"|PCR Settings
|style="background:#003E81; color:white"|PCR Settings
-
|-bgcolor="#66FFCC"
 
-
| width="200" |Annealing (°C)
 
|-bgcolor="#99CCFF"
|-bgcolor="#99CCFF"
 +
| width="200" |Annealing (°C)
 +
|-bgcolor="#f0f0f0"
|{{{tanneal}}}°
|{{{tanneal}}}°
-
|-bgcolor="#66FFCC"
 
-
|Time Elongation
 
|-bgcolor="#99CCFF"
|-bgcolor="#99CCFF"
 +
|Time Elongation
 +
|-bgcolor="#f0f0f0
|{{{telong}}}
|{{{telong}}}
-
|-bgcolor="#66FFCC"
 
-
|Number of Cycles
 
|-bgcolor="#99CCFF"
|-bgcolor="#99CCFF"
 +
|Number of Cycles
 +
|-bgcolor="#f0f0f0"
|{{{ncycles}}}
|{{{ncycles}}}
|}
|}
| width=60% |
| width=60% |
{| cellspacing="1" width=100%
{| cellspacing="1" width=100%
-
| width="200" bgcolor="#66FFCC"|Buffer (5x)
+
| width="200" bgcolor="#99CCFF"|Buffer (5x)
-
|bgcolor="#99CCFF" colspan="2"|{{{buffer}}}
+
|bgcolor="#f0f0f0" colspan="2"|{{{buffer}}}
|-
|-
-
|bgcolor="#66FFCC"|MgCl<sub>2</sub> 10 µM
+
|bgcolor="#99CCFF"|MgCl<sub>2</sub> 10 µM
-
|bgcolor="#99CCFF" colspan="2"|{{{mgcl}}}
+
|bgcolor="#f0f0f0" colspan="2"|{{{mgcl}}}
|-
|-
-
|bgcolor="#66FFCC"|dNTP 10 µM
+
|bgcolor="#99CCFF"|dNTP 10 µM
-
|bgcolor="#99CCFF" colspan="2"|{{{dntp}}}
+
|bgcolor="#f0f0f0" colspan="2"|{{{dntp}}}
|-
|-
-
|bgcolor="#66FFCC"|Oligo F 10 µM
+
|bgcolor="#99CCFF"|Oligo F 10 µM
|bgcolor="#99CCFF"|{{{fwprimername}}}
|bgcolor="#99CCFF"|{{{fwprimername}}}
|bgcolor="#99CCFF"| {{{fwprimerconc}}}
|bgcolor="#99CCFF"| {{{fwprimerconc}}}
|-
|-
-
|bgcolor="#66FFCC"|Oligo R 10 µM
+
|bgcolor="#99CCFF"|Oligo R 10 µM
-
|bgcolor="#99CCFF"|{{{rvprimername}}}
+
|bgcolor="#f0f0f0"|{{{rvprimername}}}
-
|bgcolor="#99CCFF"|{{{rvprimerconc}}}
+
|bgcolor="#f0f0f0"|{{{rvprimerconc}}}
|-
|-
-
|bgcolor="#66FFCC"|Water
+
|bgcolor="#99CCFF"|Water
|bgcolor="#99CCFF" colspan="2"|{{{water}}}
|bgcolor="#99CCFF" colspan="2"|{{{water}}}
|-
|-
-
|bgcolor="#66FFCC"|Polymerase
+
|bgcolor="#99CCFF"|Polymerase
-
|bgcolor="#99CCFF" colspan="2"|{{{polymerase}}}
+
|bgcolor="#f0f0f0" colspan="2"|{{{polymerase}}}
|-
|-
-
|bgcolor="#66FFCC"|DNA
+
|bgcolor="#99CCFF"|DNA
-
|bgcolor="#99CCFF" colspan="2"|{{{dna}}}
+
|bgcolor="#f0f0f0" colspan="2"|{{{dna}}}
|}
|}
| width=20% |
| width=20% |
{| cellspacing="1" width=100%
{| cellspacing="1" width=100%
-
| width="200" bgcolor="#66FFCC"|Expected Size
+
| width="200" bgcolor="#99CCFF"|Expected Size
|-
|-
-
|bgcolor="#99CCFF"|{{{size}}}
+
|bgcolor="#f0f0f0"|{{{size}}}
|-
|-
-
|bgcolor="#66FFCC"|Success
+
|bgcolor="#99CCFF"|Success
|-
|-
-
|bgcolor="#99CCFF"|{{{success}}}
+
|bgcolor="#f0f0f0"|{{{success}}}
|-
|-
-
|bgcolor="#66FFCC"|Image (click to enlarge)
+
|bgcolor="#99CCFF"|Image (click to enlarge)
|-
|-
-
|bgcolor="#99CCFF"|[[Image:{{{image}}}|30px]]
+
|bgcolor="#f0f0f0"|[[Image:{{{image}}}|30px]]
|-
|-
-
|bgcolor="#66FFCC"|Lane (0=ladder)
+
|bgcolor="#99CCFF"|Lane (0=ladder)
|-
|-
-
|bgcolor="#99CCFF"|{{{lane}}}
+
|bgcolor="#f0f0f0"|{{{lane}}}
|}
|}
|}
|}

Revision as of 16:54, 18 July 2008

PCR:
PCR Settings
Annealing (°C)
{{{tanneal}}}°
Time Elongation
{{{telong}}}
Number of Cycles
{{{ncycles}}}
Buffer (5x) {{{buffer}}}
MgCl2 10 µM {{{mgcl}}}
dNTP 10 µM {{{dntp}}}
Oligo F 10 µM {{{fwprimername}}} {{{fwprimerconc}}}
Oligo R 10 µM {{{rvprimername}}} {{{rvprimerconc}}}
Water {{{water}}}
Polymerase {{{polymerase}}}
DNA {{{dna}}}
Expected Size
{{{size}}}
Success
{{{success}}}
Image (click to enlarge)
[[Image:{{{image}}}|30px]]
Lane (0=ladder)
{{{lane}}}
Retrieved from "http://2008.igem.org/Team:KULeuven/Tools/PCR"