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Team:ESBS-Strasbourg/Protocols
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(New page: ==E.Coli== '''Preparation of competent cells (Mariels/Barbaras Protocol)''' Competent Bacteria <br> '''Preparation of TOP10 chemically competent cells''' [http:/...) |
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==E.Coli== | ==E.Coli== | ||
| - | '''Preparation of competent cells (Mariels/Barbaras Protocol)''' [[Competent Bacteria | Competent Bacteria]] <br> | + | '''Preparation of competent cells (Mariels/Barbaras Protocol)''' [[Team:ESBS-Strasbourg/Competent Bacteria | Competent Bacteria]] <br> |
'''Preparation of TOP10 chemically competent cells''' [http://openwetware.org/wiki/TOP10_chemically_competent_cells igem standard protocol] <br> | '''Preparation of TOP10 chemically competent cells''' [http://openwetware.org/wiki/TOP10_chemically_competent_cells igem standard protocol] <br> | ||
| - | '''Transformation of competent cells (Mariels/Barbaras Protocol)''' [[Transformation Barbara|Transformation]]<br> | + | '''Transformation of competent cells (Mariels/Barbaras Protocol)''' [[Team:ESBS-Strasbourg/Transformation Barbara|Transformation]]<br> |
==Cloning== | ==Cloning== | ||
Revision as of 12:21, 14 July 2008
E.Coli
Preparation of competent cells (Mariels/Barbaras Protocol) Competent Bacteria
Preparation of TOP10 chemically competent cells [http://openwetware.org/wiki/TOP10_chemically_competent_cells igem standard protocol]
Transformation of competent cells (Mariels/Barbaras Protocol) Transformation
Cloning
PCR
Purification of PCR product
Miniprep: plasmid amplification
Plasmid Purification
Restriction digestion
Agarose gel preparation
Dephosphorylation
Ligation
Solutions and buffers
SOB for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB]
