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Wisconsin: Lignin Project/24 June 2008
From 2008.igem.org
(Difference between revisions)
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'''Team Fungus:''' <br> | '''Team Fungus:''' <br> | ||
Ran leftovers from yesterday's PCR in 1% agrose gel<br> | Ran leftovers from yesterday's PCR in 1% agrose gel<br> | ||
| + | -correct sized bands visible in UV light <br> | ||
Used QIAquick gel extraction microcentrifuge kit and QIAquick PCR purification to get purified product<br> | Used QIAquick gel extraction microcentrifuge kit and QIAquick PCR purification to get purified product<br> | ||
<br> | <br> | ||
Used QIAprep miniprep kit to purify pET28a vectors from liquid LB + Kanamycin media that had incubated overnight | Used QIAprep miniprep kit to purify pET28a vectors from liquid LB + Kanamycin media that had incubated overnight | ||
<br> | <br> | ||
| + | Ran another touchdown PCR of both Carbon and Nitrogen limited cDNA using Herculase II polymerase <br> | ||
| + | Used QIAquick PCR purification kit to purify PCR product <br> | ||
---- | ---- | ||
'''Team Sorbitol:'''<br> | '''Team Sorbitol:'''<br> | ||
Revision as of 23:10, 14 July 2008
Team Fungus:
Ran leftovers from yesterday's PCR in 1% agrose gel
-correct sized bands visible in UV light
Used QIAquick gel extraction microcentrifuge kit and QIAquick PCR purification to get purified product
Used QIAprep miniprep kit to purify pET28a vectors from liquid LB + Kanamycin media that had incubated overnight
Ran another touchdown PCR of both Carbon and Nitrogen limited cDNA using Herculase II polymerase
Used QIAquick PCR purification kit to purify PCR product
Team Sorbitol:
