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Team:ESBS-Strasbourg/Protocols
From 2008.igem.org
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==Cloning== | ==Cloning== | ||
| - | [[Team:ESBS-Strasbourg/PCR|PCR]] <br> | + | *[[Team:ESBS-Strasbourg/PCR|PCR]] <br> |
| - | [[Team:ESBS-Strasbourg/Purification of PCR product|Purification of PCR products]] <br> | + | *[[Team:ESBS-Strasbourg/Purification of PCR product|Purification of PCR products]] <br> |
| - | [[Team:ESBS-Strasbourg/Miniprep: plasmid amplification|Miniprep:Plasmid Amplification]] <br> | + | *[[Team:ESBS-Strasbourg/Miniprep: plasmid amplification|Miniprep:Plasmid Amplification]] <br> |
| - | [[Team:ESBS-Strasbourg/Plasmid urification|Plasmid Purification]] <br> | + | *[[Team:ESBS-Strasbourg/Plasmid urification|Plasmid Purification]] <br> |
| - | [[Team:ESBS-Strasbourg/Restriction digestion|Restriction Digestion]] <br> | + | *[[Team:ESBS-Strasbourg/Restriction digestion|Restriction Digestion]] <br> |
| - | [[Team:ESBS-Strasbourg/Agarose gel preparation|Agarose Gel Preparation]] <br> | + | *[[Team:ESBS-Strasbourg/Agarose gel preparation|Agarose Gel Preparation]] <br> |
| - | [[Team:ESBS-Strasbourg/Dephosphorylation|Dephosphorylation]] <br> | + | *[[Team:ESBS-Strasbourg/Dephosphorylation|Dephosphorylation]] <br> |
| - | [[Team:ESBS-Strasbourg/Ligation|Ligation]] <br> | + | *[[Team:ESBS-Strasbourg/Ligation|Ligation]] <br> |
==Solutions and buffers== | ==Solutions and buffers== | ||
'''SOB''' for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB] <br> | '''SOB''' for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB] <br> | ||
Revision as of 22:26, 14 July 2008
E.Coli
Preparation of competent cells (Mariels/Barbaras Protocol) Competent Bacteria
Preparation of TOP10 chemically competent cells [http://openwetware.org/wiki/TOP10_chemically_competent_cells Igem Standard Protocol]
Transformation of competent cells (Mariels/Barbaras Protocol) Transformation
Cloning
- PCR
- Purification of PCR products
- Miniprep:Plasmid Amplification
- Plasmid Purification
- Restriction Digestion
- Agarose Gel Preparation
- Dephosphorylation
- Ligation
Solutions and buffers
SOB for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB]
