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Team:ESBS-Strasbourg/Protocols
From 2008.igem.org
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'''Transformation of competent cells (Mariels/Barbaras Protocol)''' [[Team:ESBS-Strasbourg/Transformation Barbara|Transformation]]<br> | '''Transformation of competent cells (Mariels/Barbaras Protocol)''' [[Team:ESBS-Strasbourg/Transformation Barbara|Transformation]]<br> | ||
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==Cloning== | ==Cloning== | ||
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*[[Team:ESBS-Strasbourg/Dephosphorylation|Dephosphorylation]] <br> | *[[Team:ESBS-Strasbourg/Dephosphorylation|Dephosphorylation]] <br> | ||
*[[Team:ESBS-Strasbourg/Ligation|Ligation]] <br> | *[[Team:ESBS-Strasbourg/Ligation|Ligation]] <br> | ||
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==Solutions and buffers== | ==Solutions and buffers== | ||
'''SOB''' for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB] <br> | '''SOB''' for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB] <br> | ||
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Revision as of 22:31, 14 July 2008
E.Coli
Preparation of competent cells (Mariels/Barbaras Protocol) Competent Bacteria
Preparation of TOP10 chemically competent cells [http://openwetware.org/wiki/TOP10_chemically_competent_cells Igem Standard Protocol]
Transformation of competent cells (Mariels/Barbaras Protocol) Transformation
Cloning |
Solutions and buffersSOB for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB] |
