Template:Team:UC Berkeley/Notebook/SC construction

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(Difference between revisions)
Line 169: Line 169:
cgtagGGATCCgggcttttgtcacaggggtaaac                                 
cgtagGGATCCgggcttttgtcacaggggtaaac                                 
</pre>
</pre>
 +
 +
---------------------------------------------------------------------------------------------------------------
 +
 +
 +
== pBca1256-K112610 ==
 +
<pre>
 +
Construction of {PhoA>} basic part
 +
       
 +
PCR ____/______ on MG1655                    (____ bp, EcoRI/BamHI)                             
 +
Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112605
 +
------------------------------------------------------
 +
cb10007    Fwd biobricking of {prepro>}
 +
cgataGAATTCatgAGATCTatgaaacaaagcactattgcactggc
 +
sc013    Reverse biobricking of {prepro>}
 +
ccagtGGATCCggcttttgtcacaggggtaaac
 +
</pre>
 +
 +
== pBca1256-K112611 ==
 +
<pre>
 +
Construction of {LamB>} basic part
 +
       
 +
PCR ____/______ on MG1655                    (____ bp, EcoRI/BamHI)                             
 +
Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112605
 +
------------------------------------------------------
 +
cb10009    Fwd biobricking of {prepro>}
 +
cgataGAATTCatgAGATCTatgatgattactctgcgcaaac
 +
sc014    Reverse biobricking of {prepro>}
 +
cgtagGGATCCcagccattgcctgagcagacattac                               
 +
</pre>
 +
 +
== pBca1256-K112612 ==
 +
<pre>
 +
Construction of {a~PhoA>} basic part K112609 (K112605 replacement)
 +
       
 +
PCR ____/______ on MG1655                    (____ bp, EcoRI/BamHI)                             
 +
Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112605
 +
------------------------------------------------------
 +
sc012      Fwd biobricking of {a~prepro>}
 +
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
 +
cb10008    Reverse biobricking of {a~prepro>}
 +
cgtagGGATCCgggcttttgtcacaggggtaaac                               
 +
</pre>
 +
 +
== pBca1256-K112613 ==
 +
<pre>
 +
Construction of {a~PhoA>} basic part K112609 (K112605 replacement)
 +
       
 +
PCR ____/______ on MG1655                    (____ bp, EcoRI/BamHI)                             
 +
Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112605
 +
------------------------------------------------------
 +
sc012      Fwd biobricking of {a~prepro>}
 +
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
 +
cb10008    Reverse biobricking of {a~prepro>}
 +
cgtagGGATCCgggcttttgtcacaggggtaaac                               
 +
</pre>
 +
 +
== pBca1256-K112614 ==
 +
<pre>
 +
Construction of {a~PhoA>} basic part K112609 (K112605 replacement)
 +
       
 +
PCR ____/______ on MG1655                    (____ bp, EcoRI/BamHI)                             
 +
Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112605
 +
------------------------------------------------------
 +
sc012      Fwd biobricking of {a~prepro>}
 +
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
 +
cb10008    Reverse biobricking of {a~prepro>}
 +
cgtagGGATCCgggcttttgtcacaggggtaaac                               
 +
</pre>
 +
 +
== pBca1256-K112615 ==
 +
<pre>
 +
Construction of {a~PhoA>} basic part K112609 (K112605 replacement)
 +
 +
PCR ____/______ on MG1655                    (____ bp, EcoRI/BamHI)                             
 +
Sub into pBca1256                            (EcoRI/BamHI, 2472+910, L)
 +
Product is pBca1256-K112605
 +
------------------------------------------------------
 +
sc012      Fwd biobricking of {a~prepro>}
 +
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
 +
cb10008    Reverse biobricking of {a~prepro>}
 +
cgtagGGATCCgggcttttgtcacaggggtaaac                               
 +
</pre>
 +
----
----

Revision as of 01:25, 25 July 2008

Contents


pBca1256-K112600 

Construction of {<AP>} basic part K112600

Wobble PCR SC001/SC002            (66 bp, EcoRI/BamHI)
Sub into pBca1256                 (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112600
----------------------------------------------------
SC001F    Forward Biobricking of <AP-tag>
cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag
SC002R    Reverse Biobricking of <AP-tag>
ccagtGGATCCttcatgccattcaattttctgcgcttcaaaaatatcg

pBca1256-K112601 

Construction of {<AP!} basic part K112601

Wobble PCR SC001/SC003            (69 bp, EcoRI/BamHI)
Sub into pBca1256                 (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112601
--------------------------------------------------
SC001    Forward Biobricking of <AP-tag!
cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag
SC003    Reverse Biobricking of <AP-tag!
ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg

pBca1256-K112602 

Construction of {<FLAG>} basic part K112602

Wobble PCR SC004/SC005            (45 bp, EcoRI/BamHI)
Sub into pBca1256                 (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112602
---------------------------------------------------
SC004     Forward Biobricking of <FLAG-tag>
cgataGAATTCatgAGATCTgactacaaggatgacgacg
SC005     Reverse Biobricking of <FLAG-tag>
ccagtGGATCCcttgtcgtcgtcatccttgtagtc

pBca1256-K112603 

Construction of {<FLAG!} basic part K112603

Wobble PCR SC004/SC006            (48 bp, EcoRI/BamHI)
Sub into pBca1256                 (EcoRI/BamHI, 2227+ 910)
Product is pBca1256-K112603
-----------------------------------------------------
SC004    Forward Biobricking of <FLAG-tag!
cgataGAATTCatgAGATCTgactacaaggatgacgacg
SC006    Reverse Biobricking of <FLAG-tag!
ccagtGGATCCttacttgtcgtcgtcatccttgtagtc

pBca1256-K112604 

Construction of {rbs_barstar>} basic part K112604

PCR mea035/sc007 on Bacillus amyloliquefaciens gen.            (320 bp, EcoRI/BamHI)
Sub into pBca1256                                              (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112604
-------------------------------
mea035    Fwd biobricking of {rbs_barstar>} 
cgataGAATTCatgAGATCTcataagaaaggagccgcacatg                              
sc007     Reverse biobricking of {rbs_barstar>}                     (Not needed...)
cgttaGGATCCagaaagtatgatggtgatgtcgc

pBca1256-K112403 

Construction of {PhoA>} basic part K112403
          
CR cb10007/cb10008 on MG1655            (95 bp, EcoRI/BamHI)                              
Sub into pBca1256                       (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112403                                            
-------------------------------------------------------------
cb10007    Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgaaacaaagcactattgcactggc
cb10008    Reverse biobricking of {prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac

pBca1256-K112404 

Construction of {LamB>} basic part K112404   
           
PCR cb10009/cb10010 on MG1655            (107 bp, EcoRI/BamHI)                                 
Sub into pBca1256                        (EcoRI/BamHI, 2472+910, L)                                               
Product is pBca1256-K112404                                           
-------------------------------------------------------------
cb10009    Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgatgattactctgcgcaaac
cb10010    Reverse biobricking of {prepro>}
cgtagGGATCCcagccattgcctgagcagacattac

pBca1256-K112605 

Construction of {a~PhoA>} basic part K112605    
         
PCR sc008/cb10008-phoA-R on MG1655            (100 bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc008      Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtaaaGtgaaacaaagcactattgcactggc
cb10008    Reverse biobricking of {a~prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac

pBca1256-K112606 

Construction of {a~LamB>} basic part K112606 
                
PCR sc009/cb10010-lamB-R on MG1655            (111 bp, EcoRI/BamHI)                               
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256- K112606
--------------------------------------------------
sc009      Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtagaatgatgattactctgcgc
cb10010    Reverse biobricking of {a~prepro>}
cgtagGGATCCcagccattgcctgagcagacattac

pBca1256-K112607 

Construction of {rbs_PhoA>} basic part K112607               

PCR sc010/cb10008-PhoA-R on MG1655            (113 bp, EcoRI/BamHI)                         
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112607
------------------------------------------------------
sc010      Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTgtacatggagaaaataaaAtgaaacaaagcac
cb10008    Reverse biobricking of {rbs_prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac

pBca1256-K112608 

Construction of {rbs_LamB>} basic part K112608   
      
PCR sc011/cb10010-lamB-R on E.coli K12 MG1655            (126 bp, EcoRI/BamHI)             
Sub into pBca1256                                        (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112608
------------------------------------------------------
sc011      Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTcaatgactcaggagatagaatg
cb10010    Reverse biobricking of {rbs_prepro>}
cgtagGGATCCcagccattgcctgagcagacattac

pBca1256-K112609 

Construction of {a~PhoA>} basic part K112609 (K112605 replacement)
         
PCR sc012/cb10008-phoA-R on MG1655            (100 bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc012      Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
cb10008    Reverse biobricking of {a~prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac


pBca1256-K112610 

Construction of {PhoA>} basic part 
         
PCR ____/______ on MG1655                     (____ bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
cb10007    Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgaaacaaagcactattgcactggc
sc013    Reverse biobricking of {prepro>}
ccagtGGATCCggcttttgtcacaggggtaaac

pBca1256-K112611 

Construction of {LamB>} basic part 
         
PCR ____/______ on MG1655                     (____ bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
cb10009    Fwd biobricking of {prepro>}
cgataGAATTCatgAGATCTatgatgattactctgcgcaaac
sc014    Reverse biobricking of {prepro>}
cgtagGGATCCcagccattgcctgagcagacattac

pBca1256-K112612 

Construction of {a~PhoA>} basic part K112609 (K112605 replacement)
         
PCR ____/______ on MG1655                     (____ bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc012      Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
cb10008    Reverse biobricking of {a~prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac

pBca1256-K112613 

Construction of {a~PhoA>} basic part K112609 (K112605 replacement)
         
PCR ____/______ on MG1655                     (____ bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc012      Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
cb10008    Reverse biobricking of {a~prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac

pBca1256-K112614 

Construction of {a~PhoA>} basic part K112609 (K112605 replacement)
         
PCR ____/______ on MG1655                     (____ bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc012      Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
cb10008    Reverse biobricking of {a~prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac

pBca1256-K112615 

Construction of {a~PhoA>} basic part K112609 (K112605 replacement)

PCR ____/______ on MG1655                     (____ bp, EcoRI/BamHI)                              
Sub into pBca1256                             (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc012      Fwd biobricking of {a~prepro>}
cgataGAATTCatgAGATCTtaaaAtgaaacaaagcactattgcactggc
cb10008    Reverse biobricking of {a~prepro>}
cgtagGGATCCgggcttttgtcacaggggtaaac


Sherine Cheung
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