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Team:Paris/July 14
From 2008.igem.org
(Difference between revisions)
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| - | + | ==Results of the transformation== | |
* Positive control : puc19 (1µl; 10pg/ml) --> No transformation | * Positive control : puc19 (1µl; 10pg/ml) --> No transformation | ||
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| - | + | ==Culture of Cells== | |
* Culture of DH5ɑ in 5ml of LB without Antibiotics | * Culture of DH5ɑ in 5ml of LB without Antibiotics | ||
| - | + | ==Preparation of medium and solutions== | |
* 1L of CCMB80 | * 1L of CCMB80 | ||
Revision as of 08:53, 16 July 2008
delete
Results of the transformation
- Positive control : puc19 (1µl; 10pg/ml) --> No transformation
- Biobricks 2007 : --> succeed of the transformation unlike the delay added in the ice
- Biobricks 2008 : --> No transformation
Conclusion : Cells are competents, because there is positive transformation
Hypothesis : problem with the protocol of DNA extraction from the registery 2008
Culture of Cells
- Culture of DH5ɑ in 5ml of LB without Antibiotics
Preparation of medium and solutions
- 1L of CCMB80
- 500ml of SOB ( 2 x 250ml)
- 500ml of SOC (2 x 250ml)
- 100ml of MgCl2 (1M)
- 150ml of CaCl2 (1M)
- 200ml of KOAC (1M)
