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Team:University of Ottawa/15 July 2008
From 2008.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
==Today on the Lab== | ==Today on the Lab== | ||
'''Tammy''' | '''Tammy''' | ||
| - | + | '''Transformation of XL10 Competent E. coli cells with pDR197:AtCKX2''' | |
:<li>1 uL Pure pDR197:AtCKX2 | :<li>1 uL Pure pDR197:AtCKX2 | ||
:<li>1 ul 1:10 dilution pDR197:AtCKX2 | :<li>1 ul 1:10 dilution pDR197:AtCKX2 | ||
| Line 10: | Line 10: | ||
:<li>3 LB Ampicillin (50 ug/mL) Plates | :<li>3 LB Ampicillin (50 ug/mL) Plates | ||
:<li>Began Incubation of transformed cells at 1:50 pm. | :<li>Began Incubation of transformed cells at 1:50 pm. | ||
| + | '''Matt''' | ||
| + | '''Inoculation of 97 - BY4742 cells''' | ||
| + | :<li> I inoculated the 97 BY4742 cells once again for glycerol stock tomorrow | ||
| + | '''PCR confirmation''' | ||
| + | :<li> Second PCR confirmation with F58, F59 was performed and gave the correct band but also gave another weird band that I don't know what it is. | ||
| + | '''Digestion of PTP2''' | ||
| + | <li> A digestion was performed on the PTP2 amplification product to create sticky ends using BamHI and xhoI and I left it in the PCR machine overnight. | ||
Revision as of 19:26, 16 July 2008
Contents |
Today on the Lab
Tammy Transformation of XL10 Competent E. coli cells with pDR197:AtCKX2
- 1 uL Pure pDR197:AtCKX2
- 1 ul 1:10 dilution pDR197:AtCKX2
- 1 ul 1:100 dilution pDR197:AtCKX2
- 100 ul XL10 E.Coli per reaction
- 900 ul LB Broth per reaction
- 3 LB Ampicillin (50 ug/mL) Plates
- Began Incubation of transformed cells at 1:50 pm.
Matt Inoculation of 97 - BY4742 cells
- I inoculated the 97 BY4742 cells once again for glycerol stock tomorrow
PCR confirmation
- Second PCR confirmation with F58, F59 was performed and gave the correct band but also gave another weird band that I don't know what it is.
Digestion of PTP2
