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EPF-Lausanne/17 July 2008
From 2008.igem.org
(New page: Results of the transformation of yesterday : All cells grew very well, except the Top10 A (X) and the Top10 - (X). We did glycerol stocks for the pUC19 and pHD1 and also minipreps of thes...) |
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All cells grew very well, except the Top10 A (X) and the Top10 - (X). | All cells grew very well, except the Top10 A (X) and the Top10 - (X). | ||
| - | We did glycerol stocks for the pUC19 and pHD1 and also minipreps of these plasmids. | + | We did glycerol stocks for the pUC19 and pHD1 and also minipreps of these plasmids. Concentrations were 111.1 and 110.0 for the pUC19 and 126.8 and 70.1 for the pHD1. |
We began a new transformation, following exactly the iGEM protocol. We took our Top10 cells, sample B. The iGEM parts used are the 4H 1018 and 8H1003. There are parts that will not be used in our project, then we used them just to practise and see the transformation efficiency. We did two transformations for each part : One part diluted in 5microliter of TE (2microliter of it was used) and the other one diluted in 15microliter of TE(6microliter of it was used). A control was made with the pUC19. | We began a new transformation, following exactly the iGEM protocol. We took our Top10 cells, sample B. The iGEM parts used are the 4H 1018 and 8H1003. There are parts that will not be used in our project, then we used them just to practise and see the transformation efficiency. We did two transformations for each part : One part diluted in 5microliter of TE (2microliter of it was used) and the other one diluted in 15microliter of TE(6microliter of it was used). A control was made with the pUC19. | ||
Revision as of 10:02, 17 July 2008
Results of the transformation of yesterday : All cells grew very well, except the Top10 A (X) and the Top10 - (X).
We did glycerol stocks for the pUC19 and pHD1 and also minipreps of these plasmids. Concentrations were 111.1 and 110.0 for the pUC19 and 126.8 and 70.1 for the pHD1.
We began a new transformation, following exactly the iGEM protocol. We took our Top10 cells, sample B. The iGEM parts used are the 4H 1018 and 8H1003. There are parts that will not be used in our project, then we used them just to practise and see the transformation efficiency. We did two transformations for each part : One part diluted in 5microliter of TE (2microliter of it was used) and the other one diluted in 15microliter of TE(6microliter of it was used). A control was made with the pUC19.
