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Team:ESBS-Strasbourg/17 July 2008
From 2008.igem.org
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- db3.1 + pxj - HS : 3*10^4 cfu/µg | - db3.1 + pxj - HS : 3*10^4 cfu/µg | ||
</pre> | </pre> | ||
| + | |||
| + | *Digestion of the plasmid of the BBa_J63002(?) clone with XbaI and SpeI | ||
| + | **1x double digestion | ||
| + | **1x step by step digestion | ||
== General == | == General == | ||
== Quote of the day == | == Quote of the day == | ||
Revision as of 10:05, 17 July 2008
Contents |
DryLab
Modeling
WetLab
- Results Transformation
- no clones for any of the three BioBricks
- clones for the LexA-BD plasmid --> useable for Miniprep
- counting:
- dh5a (Mariel) + pxj - KCM : 10^5 cfu/µg - dh5a (Mariel) + pxj - HS : 4*10^4 cfu/µg - dh5a + pxj - HS : 1.5*10^5 cfu/µg - Top10 + pxj - HS : 3.2*10^5 cfu/µg - db3.1 + pxj - HS : 3*10^4 cfu/µg
- Digestion of the plasmid of the BBa_J63002(?) clone with XbaI and SpeI
- 1x double digestion
- 1x step by step digestion
