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Team:ESBS-Strasbourg/17 July 2008
From 2008.igem.org
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| + | *The new BioBricks arrived in tubes with a sort of medium inside, we think they delivered us directly the bacteria with the BB-Plasmids inside, so we don't have to transform them on our own. Jippie!<br> | ||
*Digestion of the plasmid of the BBa_J63002(?) clone with XbaI and SpeI | *Digestion of the plasmid of the BBa_J63002(?) clone with XbaI and SpeI | ||
**1x double digestion | **1x double digestion | ||
Revision as of 07:21, 18 July 2008
Contents |
DryLab
Modeling
WetLab
- Results Transformation
- no clones for any of the three BioBricks
- clones for the LexA-BD plasmid --> useable for Miniprep
- counting:
- dh5a (Mariel) + pxj - KCM : 10^5 cfu/µg - dh5a (Mariel) + pxj - HS : 4*10^4 cfu/µg - dh5a + pxj - HS : 1.5*10^5 cfu/µg - Top10 + pxj - HS : 3.2*10^5 cfu/µg - db3.1 + pxj - HS : 3*10^4 cfu/µg
- The new BioBricks arrived in tubes with a sort of medium inside, we think they delivered us directly the bacteria with the BB-Plasmids inside, so we don't have to transform them on our own. Jippie!
- Digestion of the plasmid of the BBa_J63002(?) clone with XbaI and SpeI
- 1x double digestion
- 1x step by step digestion
