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Team:KULeuven/17 July 2008
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=== Wet Lab === | === Wet Lab === | ||
| - | The cells we transformed did not grow on the ampicillin plate. The pUC control did grow. | + | * The cells we transformed yesterday (TOP10 cells with BBa_30109 and DH5alpha with BBa_0034) did not grow on the ampicillin plate. The pUC control did grow on both plates, but the efficiency of the transformation with TOP10 cells seems to be better. |
| + | * Today we tried to transform TOP10 cells with the electroporation method. We used: | ||
| + | ** plasmid with BBa_B0034 | ||
| + | ** plasmid with BBa_Jxxxxx | ||
| + | ** plasmid with BBa_Jxxxxx (2 punches in 5ul TE-buffer) | ||
| + | ** pUC control plasmid | ||
| + | * The transformation of TOP10 cells with the heat shock method was also tried again. | ||
| + | * The protocol for making competent cells was continued. The grown TOP10 cells were inoculated in SOB broth and incubated for 8h. Then they were inoculated in a bigger volume SOB broth and incubated overnight. | ||
=== Dry Lab === | === Dry Lab === | ||
Revision as of 19:57, 17 July 2008
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Contents |
Lab Work
Wet Lab
- The cells we transformed yesterday (TOP10 cells with BBa_30109 and DH5alpha with BBa_0034) did not grow on the ampicillin plate. The pUC control did grow on both plates, but the efficiency of the transformation with TOP10 cells seems to be better.
- Today we tried to transform TOP10 cells with the electroporation method. We used:
- plasmid with BBa_B0034
- plasmid with BBa_Jxxxxx
- plasmid with BBa_Jxxxxx (2 punches in 5ul TE-buffer)
- pUC control plasmid
- The transformation of TOP10 cells with the heat shock method was also tried again.
- The protocol for making competent cells was continued. The grown TOP10 cells were inoculated in SOB broth and incubated for 8h. Then they were inoculated in a bigger volume SOB broth and incubated overnight.
Dry Lab
Modeling
Even more parameters ... Most composite parts are now realistically parametrized. Only the memory still remains and pieces of the pulse generator. This will be food for tomorrow. We have also started to put the composite parts together. Results, pictures and models (yay!) can be found in the modeling section. Things are actually looking surprisingly good :)
