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Team:KULeuven/17 July 2008
From 2008.igem.org
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* Today we tried to transform TOP10 cells with the electroporation method. We used: | * Today we tried to transform TOP10 cells with the electroporation method. We used: | ||
** plasmid with BBa_B0034 | ** plasmid with BBa_B0034 | ||
| - | ** plasmid with | + | ** plasmid with BBa_J6110 (this is a part we don't need for our project, so we don't lose any DNA during our attempts to transform cells) |
| - | ** plasmid with | + | ** plasmid with BBa_J6110 (2 punches in 5ul TE-buffer) |
** pUC control plasmid | ** pUC control plasmid | ||
| - | * | + | * There was a second attempt to transform TOP10 cells using the heat shock method from iGEM with some small changes. |
| - | * The protocol | + | * The protocol to make competent cells was continued. The grown TOP10 cells were inoculated in SOB broth and incubated for 8h. Then we inoculated them in a bigger volume SOB broth and let them incubate overnight. |
| + | Today one of our engineers, Maarten, set his first steps in the lab. We learnt him how to pipet! | ||
=== Dry Lab === | === Dry Lab === | ||
Revision as of 20:06, 17 July 2008
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Contents |
Lab Work
Wet Lab
- The cells we transformed yesterday (TOP10 cells with BBa_30109 and DH5alpha with BBa_0034) did not grow on the ampicillin plate. The pUC control did grow on both plates, but the efficiency of the transformation with TOP10 cells seems to be better.
- Today we tried to transform TOP10 cells with the electroporation method. We used:
- plasmid with BBa_B0034
- plasmid with BBa_J6110 (this is a part we don't need for our project, so we don't lose any DNA during our attempts to transform cells)
- plasmid with BBa_J6110 (2 punches in 5ul TE-buffer)
- pUC control plasmid
- There was a second attempt to transform TOP10 cells using the heat shock method from iGEM with some small changes.
- The protocol to make competent cells was continued. The grown TOP10 cells were inoculated in SOB broth and incubated for 8h. Then we inoculated them in a bigger volume SOB broth and let them incubate overnight.
Today one of our engineers, Maarten, set his first steps in the lab. We learnt him how to pipet!
Dry Lab
Modeling
Even more parameters ... Most composite parts are now realistically parametrized. Only the memory still remains and pieces of the pulse generator. This will be food for tomorrow. We have also started to put the composite parts together. Results, pictures and models (yay!) can be found in the modeling section. Things are actually looking surprisingly good :)
