Team:KULeuven/Tools/PCR

From 2008.igem.org

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|-bgcolor="#99CCFF"
|-bgcolor="#99CCFF"
|Time Elongation
|Time Elongation
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|-bgcolor="#f0f0f0"
|{{{telong}}}
|{{{telong}}}
|-bgcolor="#99CCFF"
|-bgcolor="#99CCFF"
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|bgcolor="#99CCFF"|Oligo F 10 µM
|bgcolor="#99CCFF"|Oligo F 10 µM
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|bgcolor="#99CCFF"|{{{fwprimername}}}
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|bgcolor="#f0f0f0"|{{{fwprimername}}}
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|bgcolor="#99CCFF"| {{{fwprimerconc}}}
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|bgcolor="#f0f0f0"| {{{fwprimerconc}}}
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|bgcolor="#99CCFF"|Oligo R 10 µM
|bgcolor="#99CCFF"|Oligo R 10 µM
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|bgcolor="#99CCFF"|Water
|bgcolor="#99CCFF"|Water
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|bgcolor="#99CCFF" colspan="2"|{{{water}}}
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|bgcolor="#f0f0f0" colspan="2"|{{{water}}}
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|bgcolor="#99CCFF"|Polymerase
|bgcolor="#99CCFF"|Polymerase

Latest revision as of 22:53, 18 July 2008

PCR:
PCR Settings
Annealing (°C)
{{{tanneal}}}°
Time Elongation
{{{telong}}}
Number of Cycles
{{{ncycles}}}
Buffer (5x) {{{buffer}}}
MgCl2 10 µM {{{mgcl}}}
dNTP 10 µM {{{dntp}}}
Oligo F 10 µM {{{fwprimername}}} {{{fwprimerconc}}}
Oligo R 10 µM {{{rvprimername}}} {{{rvprimerconc}}}
Water {{{water}}}
Polymerase {{{polymerase}}}
DNA {{{dna}}}
Expected Size
{{{size}}}
Success
{{{success}}}
Image (click to enlarge)
[[Image:{{{image}}}|30px]]
Lane (0=ladder)
{{{lane}}}
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