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Alberta NINT/18 July 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
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Made O/Ns from these colonies | Made O/Ns from these colonies | ||
Replated cells left at the bottom of tubes from yesterday's transformation | Replated cells left at the bottom of tubes from yesterday's transformation | ||
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Latest revision as of 23:00, 11 October 2008
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lab work (SD):
The double-plasmid cell cultures showed one-two colonies on the LB+amp+kan35 plates.
To test transformation efficiency, we will plate 150ul of XL1-B with pSB4A5+pSB4K5 on LB+amp50,
LB+kan35, and LB+amp50+kan35 plates using different amounts of plasmid and cells.
pSB4A5 (ul) pSB4K5 (ul) XL1-B (ul)
A 1.0 1.0 100
B 4.0 4.0 100
C 1.0 1.0 50
D 2.0 2.0 50
lab work (JD):
Ligations seem to have worked:
K102031: 1
K102021: 3
Negative: 0
Made O/Ns from these colonies
Replated cells left at the bottom of tubes from yesterday's transformation
