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Alberta NINT/21 July 2008
From 2008.igem.org
(Difference between revisions)
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Mini-preps of K102031.1-K102031.4 and K102021.1-K102021.4 | Mini-preps of K102031.1-K102031.4 and K102021.1-K102021.4 | ||
Sequencing reaction for all 8 and dropped off at MBSU | Sequencing reaction for all 8 and dropped off at MBSU | ||
| + | |||
| + | lab work (SD): | ||
| + | Results from 18/07/08 Transformation: | ||
| + | 1/100 4/100 1/50 2/50 | ||
| + | LB+amp50 8 20 1 10 | ||
| + | LB+kan35 6 15 0 8 | ||
| + | LB+amp50+kan35 0 0 0 0 | ||
| + | |||
| + | The concentrations of the DNA for the plasmids used must be checked as these are lower counts | ||
| + | than expected. | ||
| + | |||
| + | Inoculated a 96-well plate with overnight cultures in preparation for the lacZ assay. | ||
[[Alberta_NINT/22_July_2008 | Next entry >]] | [[Alberta_NINT/22_July_2008 | Next entry >]] | ||
Revision as of 16:10, 22 July 2008
lab work (JD):
Replated cells gave us more cells on the weekend:
K102031: 4
K102021: 4
Negative: 0
Wayne made O/N of these cells as well.
Mini-preps of K102031.1-K102031.4 and K102021.1-K102021.4
Sequencing reaction for all 8 and dropped off at MBSU
lab work (SD):
Results from 18/07/08 Transformation:
1/100 4/100 1/50 2/50
LB+amp50 8 20 1 10
LB+kan35 6 15 0 8
LB+amp50+kan35 0 0 0 0
The concentrations of the DNA for the plasmids used must be checked as these are lower counts
than expected.
Inoculated a 96-well plate with overnight cultures in preparation for the lacZ assay.
