Team:Paris/Notebook/Freezer

From 2008.igem.org

(Difference between revisions)
(→Plasmids : MiniPrep Products)
(→PCR Products)
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== PCR Products==
 
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{|
 
|- style="background: #ccccff;"
|- style="background: #ccccff;"
! colspan="5" style="background: #ccccff;" | PCR products
! colspan="5" style="background: #ccccff;" | PCR products
Line 17: Line 14:
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |P1
| style="background: #ccffcc;" |P1
-
|Lox71-FtsA-FtsZ-1
+
|  
-
|O3 Lox71-FtsA-F
+
|  
-
|O4 DEcoR1-FtsZ-R
+
|  
-
|MG1655
+
|  
-
|1537
+
|  
-
|
+
||>1kb
-
|Ø
+
-
|
+
-
|
+
-
|- style="background: #cccccc;" 
+
-
| style="background: #ccffcc;" |P2
+
-
|FtsZ-2
+
-
|O5 DEcoR1-FtsZ-F
+
-
|O2 FtsZ-R
+
-
|MG1655
+
-
|1088
+
-
|
+
-
|Ø
+
-
|
+
-
|
+
-
|- style="background: #cccccc;" 
+
-
| style="background: #ccffcc;" |P3
+
-
|DGAT-1
+
-
|27 ForDGAT1
+
-
|13 DPst1-DGAT-R
+
-
|Miniprep pKS::DGAT
+
-
|1087
+
-
|
+
-
|
+
-
|
+
-
|[[Paris/July_31|July 31]]
+
-
|- style="background: #cccccc;" 
+
-
| style="background: #ccffcc;" |P4
+
-
|DGAT-2
+
-
|12 DPst1-DGAT
+
-
|27 Rev-DGAT1
+
-
|Miniprep pKS::DGAT
+
-
|396
+
-
|
+
-
|
+
-
|
+
-
|[[Paris/July_31|July 31]]
+
-
|- style="background: #cccccc;" 
+
-
| style="background: #ccffcc;" |P5
+
-
|Lox66-DapAColi
+
-
|O6 Lox66-DapAColi-F
+
-
|O7 DapAColi-R
+
-
|MG1655
+
-
|985
+
-
|
+
-
|Ø
+
-
|
+
-
|[[Paris/July 17| July 17]], [[Paris/July 30| July 30]]
+
-
|- style="background: #cccccc;" 
+
-
| style="background: #ccffcc;" |P6
+
-
|Lox66-DapASubtilis
+
-
|O8 Lox66-DapASubt-F
+
-
|O9 DapASubt-R
+
-
|B.Subtilis 168
+
-
|979
+
-
|
+
-
|
+
-
|
+
-
|[[Paris/July 17| July 17]],[[Paris/July 27| July 27]],[[Paris/July 30| July 30]]
+
-
|- style="background: #cccccc;" 
+
-
| style="background: #ccffcc;" |P7
+
-
|Lox71-FtsZ
+
-
| O1 Lox71-FtsZ-F
+
-
| O2 FtsZ-R
+
-
|MG1655
+
-
|1260
+
-
|EcoRI site in FtsZ not mutated
+
-
|
+
-
|
+
-
|[[Paris/July 17| July 17]],[[Paris/July 30| July 30]]
+
-
|- style="background: #cccccc;" 
+
-
| style="background: #ccffcc;" |P8
+
-
|RBS-DapAColi
+
-
| O20 RBS-DapAColi-F
+
-
| O7 DapAColi-R
+
-
| MG1655
+
-
|952
+
-
|B0030-DapAColi
+
-
|
+
-
|
+
-
|[[Paris/July 23| July 23]],[[Paris/July 30| July 30]]
+
-
|- style="background: #cccccc;" 
+
-
| style="background: #ccffcc;" |P9
+
-
|RBS-DapASubtilis
+
-
| O21 RBS-DapASubt-F
+
-
| O9 DapASubt-R
+
-
|B.Subtilis 168
+
-
|946
+
-
|B0030-DapASubtilis
+
-
|
+
-
|
+
-
|[[Paris/July 23| July 23]], [[Paris/July 27| July 27]]
+
-
|- style="background: #cccccc;" 
+
-
| style="background: #ccffcc;" |P10
+
-
|Lox71-FtsA-FtsZ
+
-
|O3 Lox71-FtsA-F
+
-
|O2 FtsZ-R
+
-
|MG1655
+
-
|2580
+
-
|EcoRI site in FtsZ not mutated
+
-
|
+
-
|
+
-
|[[Paris/July 23| July 23]]
+
-
|- style="background: #cccccc;" 
+
-
| style="background: #ccffcc;" |P11
+
-
|Lox71-KmR-Lox66
+
-
| O10 Lox71-KmR-F
+
-
| O11 Lox66-KmR-R
+
-
| MP12
+
-
|1084
+
-
|
+
-
|
+
-
|
+
-
|[[Paris/July 27| July 27]],[[Paris/July 30| July 30]]
+
-
|- style="background: #cccccc;" 
+
-
| style="background: #ccffcc;" |P13
+
-
|DGAT deltaPst-1
+
-
| ForDGAT1
+
-
| Megaprimer DGAT2
+
-
| DNA plasmid (pKS::DGAT):
+
-
|1443
+
-
|
+
-
|
+
-
|
+
-
|[[Paris/August_1| August 1]]
+
-
|- style="background: #cccccc;" 
+
-
| style="background: #ccffcc;" |P14
+
-
|DGAT deltaPst-1 biobrick
+
-
| ForDGAT1bb
+
-
| RevDGAT1bb
+
-
| DGAT deltaPst1
+
-
|1504
+
-
|
+
-
|
+
-
|
+
-
|[[Paris/August_2| August 2]][[Paris/October 18| Oct. 18]]
+
-
|- style="background: #cccccc;" 
+
-
| style="background: #ccffcc;" |P15
+
-
| PacI [pTet>>lox-GFP-loxmRFP] XmaI
+
-
| O47
+
-
| O48
+
-
| [pTet>>lox-GFP-loxmRFP] L59.6
+
-
|2kb
+
-
|
+
-
|
+
-
|
+
-
|[[Paris/August_2| August 2]]
+
-
|- style="background: #cccccc;" 
+
-
| style="background: #ccffcc;" |P16
+
-
| PrefixBB-frt1-CmR-frt2-SufixBB
+
-
| O51
+
-
| O52
+
-
| pY-2C-IntC
+
-
|1kb
+
-
|
+
-
|
+
-
|
+
-
|[[Paris/October 15| Oct. 15]]
+
-
|- style="background: #cccccc;" 
+
-
| style="background: #ccffcc;" |P17
+
-
| PrefixBB-frt1-CmR-frt2-T1T2-SufixBB
+
-
| O51
+
-
| O53
+
-
| pY-2C-IntC
+
-
|>1kb
+
|
|
|
|

Revision as of 12:42, 22 July 2008

|- style="background: #ccccff;" ! colspan="5" style="background: #ccccff;" | PCR products |- style="background: #ccccff; text-align: center;" |width=5%| Number |width=10%| Name |width=12.5%| Oligo F |width=12.5%| Oligo R |width=10%| Matrix |width=5%| Length |width=25%| Comments |width=5%| Product Left ? |width=5%| []ng/µL |width=5%| Date |- style="background: #cccccc;" | style="background: #ccffcc;" |P1 | | | | | ||>1kb | | | | Oct. 18 |}

Plasmids : MiniPrep Products

Plasmids: MiniPrep Products
Number Name Description Products in use Date
MP1 [http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002] Strong constitutive promoter in J61002(clones 1 + 2) pJ23100 July 15, 2007; Aug. 17, 2007
MP2 [http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002] Medium constitutive promoter in J61002 (clones 1 + 2) pJ23100 MP2.2 July 15, 2007; Aug. 17, 2007
MP3 [http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1Ak3 pSB1AK3] Double terminator (clones 1 + 2) B0015B0015 MP3.3, MP3.4 August 14, 2007
MP4 [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] AraC pBAD (clones 1 + 2) pJ23100 July 16, 2007
MP5 [http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Strong RBS (clones 1 + 2) B0030 July 18, 2007
MP6 [http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] ECFP (RBS+LVA+Term) (clones 1 + 2) B0030B0030B0030B0030 July 18, 2007; July 24, 2007
MP7 [http://partsregistry.org/Part:BBa_E0241 E0241] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] PoPs to GFP converter (clones 1 + 2) B0030B0030B0030 MP7'.1 July 18, 2007; July 24, 2007
MP8 <bbpart>BBa_E0840</bbpart> in <bbpart>pSB1A2</bbpart> gfp tri-part; strong rbs (clones 1 + 2) <partinfo>E0840 SpecifiedComponents</partinfo> July 18
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