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Team:University of Ottawa/19 July 2008
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:'''Ligation''' | :'''Ligation''' | ||
::<li>Spiked my overnight ligation with 1 uL ATP and 1 uL enzyme. | ::<li>Spiked my overnight ligation with 1 uL ATP and 1 uL enzyme. | ||
| - | :''Ligation of Atcre''' | + | :'''Ligation of Atcre''' |
::<li> Began ligation of Chris's Atcre component in ~50 uL reaction | ::<li> Began ligation of Chris's Atcre component in ~50 uL reaction | ||
| + | '''Matt''' | ||
| + | :'''Inoculation''' | ||
| + | ::<li>Today I took out the inoculated bacteria from Friday and put them in the -4 fridge (PTP2). All of the bacteria grew and the control was clean. | ||
| + | ::<li>I also inoculated both BY4742 WT and YPH500 WT for Katy for a Mass Spec test. | ||
| + | :'''PCR''' | ||
| + | ::<li>I also performed a PCR cleanup of the 600/01 plasmids after I confirmed them on a Gel. These plasmids will now be ready for integration next week. | ||
Revision as of 14:58, 22 July 2008
Contents |
Today in the lab
Dan
- Ligation
- Spiked my overnight ligation with 1 uL ATP and 1 uL enzyme.
- Ligation of Atcre
- Began ligation of Chris's Atcre component in ~50 uL reaction
Matt
- Inoculation
- Today I took out the inoculated bacteria from Friday and put them in the -4 fridge (PTP2). All of the bacteria grew and the control was clean.
- I also inoculated both BY4742 WT and YPH500 WT for Katy for a Mass Spec test.
- PCR
- I also performed a PCR cleanup of the 600/01 plasmids after I confirmed them on a Gel. These plasmids will now be ready for integration next week.
