User:University of Washington/24 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: ---- Back to Team:University_of_Washington/Notebook#Notebook) |
|||
Line 1: | Line 1: | ||
+ | ==LuxR from AraC and TetR== | ||
+ | |||
+ | - miniprepped mutated AraC (reaction1 x 4) (reaction2 x 4) in 30 ul EB. | ||
+ | |||
+ | - ordered sequencing for mutated AraC with forward primers. | ||
+ | |||
+ | - ran gel for R0080 (miniprepped yesterday) to see if AraC DNA in 6 tubes had same length. All except tube two appeared to be around 2 kb(as expected), so they were combined into one. | ||
Revision as of 22:19, 24 July 2008
LuxR from AraC and TetR
- miniprepped mutated AraC (reaction1 x 4) (reaction2 x 4) in 30 ul EB.
- ordered sequencing for mutated AraC with forward primers.
- ran gel for R0080 (miniprepped yesterday) to see if AraC DNA in 6 tubes had same length. All except tube two appeared to be around 2 kb(as expected), so they were combined into one.
Back to Team:University_of_Washington/Notebook#Notebook