User:University of Washington/25 July 2008

From 2008.igem.org

(Difference between revisions)
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==LuxR from AraC and TetR==
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-Received primers to biobrick Elowitz's plasmid.
 +
 +
-Did PCR amplification on Elowitz's plasmid.
 +
 +
<table>
 +
<tr>
 +
  <th>reagents</th><th>amount per reaction(ul)</th>
 +
</tr>
 +
<tr>
 +
  <td>sterile dH2O</td><td>34.5</td>
 +
</tr>
 +
<tr>
 +
  <td>buffer for Taq (-MgCl2)</td><td>5</td>
 +
</tr>
 +
<tr>
 +
  <td>10 mM dNTPs</td><td>1</td>
 +
</tr>
 +
<tr>
 +
  <td>MgCl2</td><td>2</td>
 +
</tr>
 +
<tr>
 +
  <td>F primer(#436)</td><td>2.5</td>
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</tr>
 +
<tr>
 +
  <td>R primer(#436)</td><td>2.5</td>
 +
</tr>
 +
<tr>
 +
  <td>Taq</td><td>0.5</td>
 +
</tr>
 +
<tr>
 +
  <td>DNA template</td><td>2</td>
 +
</tr>
 +
</table>
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Back to [[Team:University_of_Washington/Notebook#Notebook]]
Back to [[Team:University_of_Washington/Notebook#Notebook]]

Revision as of 20:54, 25 July 2008

LuxR from AraC and TetR

-Received primers to biobrick Elowitz's plasmid.

-Did PCR amplification on Elowitz's plasmid.

reagentsamount per reaction(ul)
sterile dH2O34.5
buffer for Taq (-MgCl2)5
10 mM dNTPs1
MgCl22
F primer(#436)2.5
R primer(#436)2.5
Taq0.5
DNA template2


Back to Team:University_of_Washington/Notebook#Notebook

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