Beijing Normal/21 July 2008

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(Difference between revisions)
(New page: Results: The transformation of pSB1A3 is unsuccessful, however the situation of pSB1AT3 is no better: just 20~30 colonies. The steps as follows: 1.cut douwn the sports...)
 
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Results:
Results:
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       The transformation of pSB1A3 is unsuccessful, however the situation of pSB1AT3 is no better: just 20~30 colonies.
+
       The transformation of pSB1A3 is unsuccessful,
 +
      however the situation of pSB1AT3 is no better: just 20~30 colonies.
       The steps as follows:
       The steps as follows:
       1.cut douwn the sports stricktly following the instructions provided and we do spin it.  
       1.cut douwn the sports stricktly following the instructions provided and we do spin it.  
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       7 37 degree incubate for more than 60mins
       7 37 degree incubate for more than 60mins
       8 plate the incubated cell culture on the plate containing amp
       8 plate the incubated cell culture on the plate containing amp
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       9 incubate the plate at 37 degree for 12~14h\
+
       9 incubate the plate at 37 degree for 12~14h
Questions:
Questions:
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   2 Is 5ul TE enough for u to elute the plamids? Or the solution is all absorbed by the
   2 Is 5ul TE enough for u to elute the plamids? Or the solution is all absorbed by the
   the soaked paper?
   the soaked paper?
-
   3 What does the instruction mean by 10% bleach? We have several kinds of bleach in the lab and are confused about which is proper.
+
   3 What does the instruction mean by 10% bleach?  
 +
  We have several kinds of bleach in the lab and are confused about which is proper.

Latest revision as of 04:52, 23 July 2008

Results:

      The transformation of pSB1A3 is unsuccessful,
      however the situation of pSB1AT3 is no better: just 20~30 colonies.
      The steps as follows:
      1.cut douwn the sports stricktly following the instructions provided and we do spin it. 
      2. add all the TE containing the plasmids to 50ul competence cell (Top10, tested before expet) 
      3 stock in the 4 degree frigde for 30mins
      4 42 degree heat shock
      5 4 degree stock for 5~6 mins
      6 add 300ul SOB.
      7 37 degree incubate for more than 60mins
      8 plate the incubated cell culture on the plate containing amp
      9 incubate the plate at 37 degree for 12~14h

Questions:

  However, we wanna to ask other teams members:
  1 When u eject the sport into the tube, will the colour turn pink?
  2 Is 5ul TE enough for u to elute the plamids? Or the solution is all absorbed by the
  the soaked paper?
  3 What does the instruction mean by 10% bleach? 
  We have several kinds of bleach in the lab and are confused about which is proper.