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Minnesota/16 July 2008
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|2. '''Move Dual Promoter Plates from incubator to fridge'''. This will stop over growth and mutations before picking into 2mL cultures is performed later on. | |2. '''Move Dual Promoter Plates from incubator to fridge'''. This will stop over growth and mutations before picking into 2mL cultures is performed later on. | ||
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| - | |3. '''Transform paper DNA from iGEM notebook:''' (1) RFP, (2) TetR promoter, (3) Terminator, (4) MCherry. Follow iGEM handout for details on paper transformations into competent cells. | + | |3. '''Transform paper DNA from iGEM notebook:''' (1) RFP, (2) TetR promoter, (3) Terminator, (4) MCherry. Follow iGEM handout for details on paper transformations into DH5alpha competent cells. |
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|[[Image:TransformationTetR.jpg|thumb|left|600px|Example of Transformation using TetR Promoter Gene]] | |[[Image:TransformationTetR.jpg|thumb|left|600px|Example of Transformation using TetR Promoter Gene]] | ||
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|4. '''Sequencing Results:''' Sequencing results returned poor results. Redo/rework on sequences. | |4. '''Sequencing Results:''' Sequencing results returned poor results. Redo/rework on sequences. | ||
Revision as of 19:07, 23 July 2008
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| 1. Plasmid Prep 2mL Ligated Product cultures from 07-14-2008. Follow the QIA prep and purify steps as seen in on 07-14-2008. |
| 2. Move Dual Promoter Plates from incubator to fridge. This will stop over growth and mutations before picking into 2mL cultures is performed later on. |
| 3. Transform paper DNA from iGEM notebook: (1) RFP, (2) TetR promoter, (3) Terminator, (4) MCherry. Follow iGEM handout for details on paper transformations into DH5alpha competent cells. |
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| 4. Sequencing Results: Sequencing results returned poor results. Redo/rework on sequences. |
