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Team:BCCS-Bristol/Calendar-Notebook/15 July 2008
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Rodgerread (Talk | contribs) (New page: Made SOC medium in preparation for Biobrick transformation this consisted of {| {{table}} | align="center" style="background:#f0f0f0;"|'''Trypton''' | align="center" style="background:#f...) |
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then freeze at -20oC. this solution is from Molecular Cloning 3 A.2 SOB/SOC Medium. | then freeze at -20oC. this solution is from Molecular Cloning 3 A.2 SOB/SOC Medium. | ||
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| + | had a go at making wells as in the diagram (UPLOAD DIAGRAM), needs diff glue | ||
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| + | Recorded motility comparison, turns out we messed up and tried to grow MG1655 on streptomycin, therefore we made a repeat of this experiment | ||
Revision as of 11:43, 24 July 2008
Made SOC medium in preparation for Biobrick transformation this consisted of
| Trypton | 2g |
| Yeast Extract | 0.5g |
| NaCl | 0.05g |
in 95ml of water, then autoclaved. after this add this:
| MgCl2 | 0.5ml 2M solution |
| Glucose | 2ml 1M |
then freeze at -20oC. this solution is from Molecular Cloning 3 A.2 SOB/SOC Medium.
had a go at making wells as in the diagram (UPLOAD DIAGRAM), needs diff glue
Recorded motility comparison, turns out we messed up and tried to grow MG1655 on streptomycin, therefore we made a repeat of this experiment
