Team:BCCS-Bristol/Calendar-Notebook/1 July 2008

From 2008.igem.org

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This is a page for any solutions
This is a page for any solutions
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Media for Plates'''
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| align="center" style="background:#f0f0f0;"|'''0.3% 400ml'''
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| align="center" style="background:#f0f0f0;"|'''0.15% 150ml'''
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| align="center" style="background:#f0f0f0;"|'''0.5% 150ml'''
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|-
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| ||||||
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|-
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| Bactotryptone||4g||1.5g||1.5g
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|-
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| NaCl||2g||0.75g||0.75g
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|-
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| BactoAgar||1.2g||0.225g||0.75g
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|-
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|
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|}
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in addition did  a streak out for master colonies of MG1655 in LB from table below overnight at 37oC
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''10ml LB'''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Yeast Extract||0.05
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|-
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| Tryptone||0.1
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|-
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| Nacl||0.1
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|-
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|
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|}
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SOC medium in preparation for Biobrick transformation this consisted of  
SOC medium in preparation for Biobrick transformation this consisted of  

Revision as of 11:46, 24 July 2008

This is a page for any solutions

Media for Plates 0.3% 400ml 0.15% 150ml 0.5% 150ml
Bactotryptone4g1.5g1.5g
NaCl2g0.75g0.75g
BactoAgar1.2g0.225g0.75g

in addition did a streak out for master colonies of MG1655 in LB from table below overnight at 37oC

10ml LB '
Yeast Extract0.05
Tryptone0.1
Nacl0.1



SOC medium in preparation for Biobrick transformation this consisted of

Trypton 2g
Yeast Extract0.5g
NaCl0.05g

in 95ml of water, then autoclaved. after this add this:

MgCl2 0.5ml 2M solution
Glucose2ml 1M

then freeze at -20oC. this solution is from Molecular Cloning 3 A.2 SOB/SOC Medium.

Retrieved from "http://2008.igem.org/Team:BCCS-Bristol/Calendar-Notebook/1_July_2008"