Team:BCCS-Bristol/Calendar-Notebook/21 July 2008

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Revision as of 11:49, 24 July 2008

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OD measurement

The OD of both strains was measured over a day so that we would know whether the bacteria were in their log phase in any experiments requiring innoculation of bacteria.

A, B and C show 100, 500 and 200µl innoculations respectively.

Time (minutes)				OD600 (1.5 diluted)
		MG1655				MC1000
	A	B	C		A	B	C
0	0.0016	0.0042	0.017		0.0016	0.0044	0.0174
60	0.008	0.013	0.073		0.006	0.017	0.068
80	0.011	0.052	0.109		0.009	0.046	0.123
100	0.019	0.059	0.142		0.017	0.067	0.167
120	0.029	0.076	0.169		0.024	0.083	0.183
140	0.039	0.095	0.213		0.039	0.144	0.226
160	0.063	0.136	0.26		0.053	0.147	0.275
180	0.074	0.166	0.276		0.069	0.186	0.298
200	0.105	0.207	0.301		0.089	0.22	0.316
220	0.134	0.25	0.355		0.116	0.268	0.331
240	0.178	0.276	0.386		0.151	0.293	0.341
260	0.233	0.329	0.419		0.198	0.325	0.372
280	0.239	0.334	0.435		0.203	0.326	0.375
300	0.263	0.363	0.473		0.238	0.34	0.388
320	0.296	0.391	0.491		0.27	0.364	0.412
340	0.319	0.425	0.511		0.29	0.378	0.429
360	0.32	0.435	0.512		0.294	0.382	0.432
380	0.392	0.542	0.514		0.311	0.434	0.438
400	0.435	0.549	0.588		0.348	0.457	0.497
420	0.47	0.52	0.487		0.382	0.462	0.504
440	0.483	0.586	0.633		0.477	0.437	0.516
460	0.498	0.482	0.629		0.411	0.484	0.517
480	0.574	0.643	0.721		0.451	0.516	0.524
500	0.607	0.66	0.73		0.455	0.514	0.515
520	0.65	0.673	0.723		0.453	0.522	0.523

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