Team:BCCS-Bristol/Calendar-Notebook/22 July 2008

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Started using the beads, aspartate and bacteria in wells. all in 0.3% agar. focused on one bead under microscope, didn't move after 30 minutes.
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Carried on with biobrick transformation, tranformed the DNA from spot into DH5 alpha cells along with pUC19 as a control.
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continued with chemotaxis experiment, set up gradient over 8 hours of diffusion and innoculated with both MC1000 and MG1655 in agar in wells as before. (18.7)

Revision as of 15:24, 24 July 2008


Started using the beads, aspartate and bacteria in wells. all in 0.3% agar. focused on one bead under microscope, didn't move after 30 minutes.

Carried on with biobrick transformation, tranformed the DNA from spot into DH5 alpha cells along with pUC19 as a control.

continued with chemotaxis experiment, set up gradient over 8 hours of diffusion and innoculated with both MC1000 and MG1655 in agar in wells as before. (18.7)