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Team:BCCS-Bristol/Calendar-Notebook/22 July 2008
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| + | ==Bead experiment 1== | ||
| + | It was started using the beads, aspartate and bacteria in 0.3 % agar within the glass slide chambers. One bead was focused under the microscope; it didn't move after 30 minutes probably due to the observation that the bacteria were in the bottom layer and the beads on top of the motility medium in didn`t seem to get in contact. | ||
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| + | ==BioBrick Transformation== | ||
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| + | Carried on with biobrick transformation of the [http://partsregistry.org/Part:BBa_E0240 GFP generator]. The DNA was transformed into ''E. coli'' DH5α cells along with pUC19 as a control using chemical competent cells and heat shock. | ||
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continued with chemotaxis experiment, set up gradient over 8 hours of diffusion and innoculated with both MC1000 and MG1655 in agar in wells as before. (18.7) | continued with chemotaxis experiment, set up gradient over 8 hours of diffusion and innoculated with both MC1000 and MG1655 in agar in wells as before. (18.7) | ||
Revision as of 13:05, 8 August 2008
Bead experiment 1
It was started using the beads, aspartate and bacteria in 0.3 % agar within the glass slide chambers. One bead was focused under the microscope; it didn't move after 30 minutes probably due to the observation that the bacteria were in the bottom layer and the beads on top of the motility medium in didn`t seem to get in contact.
BioBrick Transformation
Carried on with biobrick transformation of the [http://partsregistry.org/Part:BBa_E0240 GFP generator]. The DNA was transformed into E. coli DH5α cells along with pUC19 as a control using chemical competent cells and heat shock.
continued with chemotaxis experiment, set up gradient over 8 hours of diffusion and innoculated with both MC1000 and MG1655 in agar in wells as before. (18.7)
