Team:BCCS-Bristol/Calendar-Notebook/21 July 2008

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Revision as of 16:11, 24 July 2008

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OD measurement

The OD of both strains was measured over a day so that we would know whether the bacteria were in their log phase in any experiments requiring innoculation of bacteria.

A, B and C show 100, 500 and 200µl innoculations respectively.

Time (minutes)				OD₆₀₀ (1.5 diluted)
		MG1655				MC1000
	A	B	C		A	B	C
0	0.0016	0.0042	0.017		0.0016	0.0044	0.0174
60	0.008	0.013	0.073		0.006	0.017	0.068
80	0.011	0.052	0.109		0.009	0.046	0.123
100	0.019	0.059	0.142		0.017	0.067	0.167
120	0.029	0.076	0.169		0.024	0.083	0.183
140	0.039	0.095	0.213		0.039	0.144	0.226
160	0.063	0.136	0.26		0.053	0.147	0.275
180	0.074	0.166	0.276		0.069	0.186	0.298
200	0.105	0.207	0.301		0.089	0.22	0.316
220	0.134	0.25	0.355		0.116	0.268	0.331
240	0.178	0.276	0.386		0.151	0.293	0.341
260	0.233	0.329	0.419		0.198	0.325	0.372
280	0.239	0.334	0.435		0.203	0.326	0.375
300	0.263	0.363	0.473		0.238	0.34	0.388
320	0.296	0.391	0.491		0.27	0.364	0.412
340	0.319	0.425	0.511		0.29	0.378	0.429
360	0.32	0.435	0.512		0.294	0.382	0.432
380	0.392	0.542	0.514		0.311	0.434	0.438
400	0.435	0.549	0.588		0.348	0.457	0.497
420	0.47	0.52	0.487		0.382	0.462	0.504
440	0.483	0.586	0.633		0.477	0.437	0.516
460	0.498	0.482	0.629		0.411	0.484	0.517
480	0.574	0.643	0.721		0.451	0.516	0.524
500	0.607	0.66	0.73		0.455	0.514	0.515
520	0.65	0.673	0.723		0.453	0.522	0.523

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Chemotaxis experiment for MG1655 showed no real difference btween the control (water) and the aspartate. may have been due to higher agar concentration caused by an unusually large water loss in autoclaving, causing high concentrations of agar.

Started on the transformation of bacteria with biobrick from paper, punched out hole and put in TE buffer (below) following protocol on igem sheet.

Chemotaxis experiment with 20% aspartate repeated.

DMSO stocks of master cultures made as back up in the -80 ^o C freezer.

@@ Line 29: / Line 29: @@
 | align="center" |'''''
 | align="center" |'''''
-| align="center" |'''OD600 (1.5 diluted)'''
+| align="center" |'''OD<sub>600</sub> (1.5 diluted)'''
 | align="center" |'''''
 | align="center" |'''''
@@ Line 101: / Line 101: @@
 Chemotaxis experiment with 20% aspartate repeated.
-DMSO stocks of master cultures made as back up in the -80oC freezer.
+DMSO stocks of master cultures made as back up in the -80 <sup>o</sup> C freezer.