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Team:ESBS-Strasbourg/24 July 2008
From 2008.igem.org
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== WetLab == | == WetLab == | ||
| + | ''Manuel'' | ||
| + | *tetR, lacI PCR on BB (Primers, VFPrimers) | ||
| + | *Pfu Pol PCR on pGEX lacI containing plasmid | ||
''Sandra'' | ''Sandra'' | ||
* Maxiprep of plasmid p10101A2 with final amount of 70 µg and a good quality (ratio 260/280 = 1,7)<br> | * Maxiprep of plasmid p10101A2 with final amount of 70 µg and a good quality (ratio 260/280 = 1,7)<br> | ||
Revision as of 13:58, 28 July 2008
Contents |
DryLab
Modeling
WetLab
Manuel
- tetR, lacI PCR on BB (Primers, VFPrimers)
- Pfu Pol PCR on pGEX lacI containing plasmid
Sandra
- Maxiprep of plasmid p10101A2 with final amount of 70 µg and a good quality (ratio 260/280 = 1,7)
=> find them in the -20°C fridge in our lab in tubes with different concentrations
Paul
Digestion of tetR/lacI/c1 plasmids, in order to check if the biobricks are really there. Used of EcoR1 and Spe1.
=> no results, probably not enough DNA for the digestion. To redo next day.
General
- Animation (MM)
