Alberta NINT/25 July 2008

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(New page: < Back to notebook < Previous entry lab work (SD): Some yellow color developed overnight so 50 ul of Na2CO3 ...)
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lab work (SD):
lab work (SD):
             Some yellow color developed overnight so 50 ul of Na2CO3 was added to stop the reaction. The A550  
             Some yellow color developed overnight so 50 ul of Na2CO3 was added to stop the reaction. The A550  
-
             and A420 readings were taken.
+
             and A420 readings were taken. Miller units were calculated using the formula
 +
                    Miller Units = 1000 x [(A420 - 1.75 x A550] / (T x V x 4 x A600)
 +
                where A420 is detection of yellow
 +
                      A550 is detection of cell debris
 +
                      T = time of reaction in minutes
 +
                      V = volume of culture used in the assay in mLs
 +
                      A600 is detection of cell density
[[Alberta_NINT/28_July_2008 | Next entry >]]
[[Alberta_NINT/28_July_2008 | Next entry >]]

Revision as of 19:24, 25 July 2008

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lab work (SD): 

            Some yellow color developed overnight so 50 ul of Na2CO3 was added to stop the reaction. The A550 
            and A420 readings were taken.  Miller units were calculated using the formula
                   Miller Units = 1000 x [(A420 - 1.75 x A550] / (T x V x 4 x A600)
                where A420 is detection of yellow
                      A550 is detection of cell debris
                      T = time of reaction in minutes
                      V = volume of culture used in the assay in mLs
                      A600 is detection of cell density

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