User:University of Washington/25 July 2008
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-Did PCR amplification on Elowitz's plasmid. | -Did PCR amplification on Elowitz's plasmid. | ||
+ | *Reaction Set-Up | ||
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<tr> | <tr> |
Revision as of 21:15, 25 July 2008
LuxR from AraC and TetR
-Received primers to biobrick Elowitz's plasmid.
-Did PCR amplification on Elowitz's plasmid.
- Reaction Set-Up
reagents | amount per reaction(ul) |
---|---|
sterile dH2O | 34.5 |
buffer for Taq (-MgCl2) | 5 |
10 mM dNTPs | 1 |
MgCl2 | 2 |
F primer(#436) | 2.5 |
R primer(#436) | 2.5 |
Taq | 0.5 |
DNA template | 2 |
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