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User:University of Washington/25 July 2008
From 2008.igem.org
(Difference between revisions)
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| - | <td rowspan=3>2</td><td rowspan=3> | + | <td rowspan=3>2</td><td rowspan=3>30</td><td>95°C</td><td>30 seconds</td> |
</tr> | </tr> | ||
<tr align=center> | <tr align=center> | ||
| - | <td> | + | <td>50°C</td><td>1 min</td> |
</tr> | </tr> | ||
<tr align=center> | <tr align=center> | ||
| - | <td> | + | <td>72°C</td><td>1 min</td> |
</tr> | </tr> | ||
<tr align=center> | <tr align=center> | ||
| - | <td>3</td><td>1</td><td> | + | <td>3</td><td>1</td><td>72°C</td><td>7 minutes</td> |
| + | </tr> | ||
| + | <tr align=center> | ||
| + | <td>4</td><td>-</td><td>4°C</td><td>infinite</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
| + | |||
| + | |||
---- | ---- | ||
Back to [[Team:University_of_Washington/Notebook#Notebook]] | Back to [[Team:University_of_Washington/Notebook#Notebook]] | ||
Revision as of 21:29, 25 July 2008
LuxR from AraC and TetR
-Received primers to biobrick Elowitz's plasmid.
-Did PCR amplification on Elowitz's plasmid.
- Reaction Set-Up
| reagents | amount per reaction(ul) |
|---|---|
| sterile dH2O | 34.5 |
| buffer for Taq (-MgCl2) | 5 |
| 10 mM dNTPs | 1 |
| MgCl2 | 2 |
| F primer(#436) | 2.5 |
| R primer(#436) | 2.5 |
| Taq | 0.5 |
| DNA template | 2 |
- Thermocycling
| Segment | Cycles | Temperature | Time |
|---|---|---|---|
| 1 | 1 | 95°C | 1 minute |
| 2 | 30 | 95°C | 30 seconds |
| 50°C | 1 min | ||
| 72°C | 1 min | ||
| 3 | 1 | 72°C | 7 minutes |
| 4 | - | 4°C | infinite |
Back to Team:University_of_Washington/Notebook#Notebook
