User:University of Washington/25 July 2008

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   <td rowspan=3>2</td><td rowspan=3>30</td><td align=center>95°C</td><td align=center>30 seconds</td>  
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Revision as of 21:38, 25 July 2008

LuxR from AraC and TetR

-Received primers to biobrick Elowitz's plasmid.

-Did PCR amplification on Elowitz's plasmid.

  • Reaction Set-Up
reagentsamount per reaction(ul)
sterile dH2O34.5
buffer for Taq (-MgCl2)5
10 mM dNTPs1
MgCl22
F primer(#436)2.5
R primer(#436)2.5
Taq0.5
DNA template2
  • Thermocycling
SegmentCyclesTemperatureTime
1195°C1 minute
23095°C30 seconds
50°C1 min
72°C1 min
3172°C7 minutes
4-4°Cinfinite

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