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Team:ESBS-Strasbourg/Concentrations Measurements
From 2008.igem.org
(Difference between revisions)
| Line 4: | Line 4: | ||
2µL of the solution <br> | 2µL of the solution <br> | ||
400µL Water <br> | 400µL Water <br> | ||
| + | |||
step2: put lambda=260nm <br> | step2: put lambda=260nm <br> | ||
| + | |||
step3: do the blank with the same water you used for dilution <br> | step3: do the blank with the same water you used for dilution <br> | ||
| + | |||
step4: Do the conversion absorbance->concentrations <br> | step4: Do the conversion absorbance->concentrations <br> | ||
| Line 15: | Line 18: | ||
=>Multiply your DO per 50, and then per 200 for the dilution factor. Then you have your DNA concentrations in ng/µL. <br> | =>Multiply your DO per 50, and then per 200 for the dilution factor. Then you have your DNA concentrations in ng/µL. <br> | ||
| - | '''By nanodrop''' | + | |
| + | |||
| + | '''2)By nanodrop''' | ||
Ask Maria to use it at the Pharma faculty. You only need 1µL of your solution. <br> | Ask Maria to use it at the Pharma faculty. You only need 1µL of your solution. <br> | ||
Revision as of 13:07, 29 July 2008
1)by spectrophotometer
step1: Prepare your sample:
2µL of the solution
400µL Water
step2: put lambda=260nm
step3: do the blank with the same water you used for dilution
step4: Do the conversion absorbance->concentrations
1DO = 50µgDNA/mL = 50ng/µL
=>Multiply your DO per 50, and then per 200 for the dilution factor. Then you have your DNA concentrations in ng/µL.
2)By nanodrop
Ask Maria to use it at the Pharma faculty. You only need 1µL of your solution.
