Team:Paris/Notebook/Oligo

From 2008.igem.org

(Difference between revisions)
Line 15: Line 15:
|width=45%| Comments
|width=45%| Comments
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
-
| style="background: #ccffcc;" |O100
+
| style="background: #D4E2EF;" |O100
| FlgA-F
| FlgA-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCATATCTCCTCCGCAGGTATCAAAAT
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCATATCTCCTCCGCAGGTATCAAAAT
Line 22: Line 22:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O101
+
| style="background: #D4E2EF;" | O101
| FlgA-R
| FlgA-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAACAGTATCGCGATGATCGCCACGCTACGT
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAACAGTATCGCGATGATCGCCACGCTACGT
Line 29: Line 29:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O102
+
| style="background: #D4E2EF;" | O102
| FlgB-F
| FlgB-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGACAGTATCGCGATGATCGCCACGCTACG
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGACAGTATCGCGATGATCGCCACGCTACG
Line 36: Line 36:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O103
+
| style="background: #D4E2EF;" | O103
| FlgB-R
| FlgB-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGCATATCTCCTCCGCAGGTATCAAAATT
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGCATATCTCCTCCGCAGGTATCAAAATT
Line 43: Line 43:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O104
+
| style="background: #D4E2EF;" | O104
| FlgK-F
| FlgK-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCAGATGAAATCGATAAGCGACAAGG
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCAGATGAAATCGATAAGCGACAAGG
Line 50: Line 50:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O105
+
| style="background: #D4E2EF;" | O105
| FlgK-R
| FlgK-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAAACGCCGCCTGGGCCGCGTTCAGTCCGCT
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAAACGCCGCCTGGGCCGCGTTCAGTCCGCT
Line 57: Line 57:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O106
+
| style="background: #D4E2EF;" | O106
| FlgM-F
| FlgM-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCGGCGTTGTTGATGCAGATGGGAATA
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCGGCGTTGTTGATGCAGATGGGAATA
Line 64: Line 64:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O107
+
| style="background: #D4E2EF;" | O107
| FlgM-R
| FlgM-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACGAAGTGCGATCAATACTCATGGTTTATT
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACGAAGTGCGATCAATACTCATGGTTTATT
Line 71: Line 71:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O108
+
| style="background: #D4E2EF;" | O108
| FlhB-F
| FlhB-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACGTCATATCAGGCGGTCTGATAAGG
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACGTCATATCAGGCGGTCTGATAAGG
Line 78: Line 78:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O109
+
| style="background: #D4E2EF;" | O109
| FlhB-R
| FlhB-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTTTTGTCGTCGCTCTCGTCAGACACGTC
| GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTTTTGTCGTCGCTCTCGTCAGACACGTC
Line 85: Line 85:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O110
+
| style="background: #D4E2EF;" | O110
| FlhDC-Uri-F
| FlhDC-Uri-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGTATGTGCGTGTAGTGACGAGTACAG
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGTATGTGCGTGTAGTGACGAGTACAG
Line 92: Line 92:
|Don't amplify the both OmpR binding site
|Don't amplify the both OmpR binding site
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O111
+
| style="background: #D4E2EF;" | O111
| FlhDC-Total-F
| FlhDC-Total-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTCATTTTTGCTTGCTAGCGTACGGAAAA
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTCATTTTTGCTTGCTAGCGTACGGAAAA
Line 99: Line 99:
|Amplify the both OmpR binding site
|Amplify the both OmpR binding site
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O112
+
| style="background: #D4E2EF;" | O112
| FlhDC(rbs)-R
| FlhDC(rbs)-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTATCCCACCCAGAATAACCAACTTTAT
| GTTTCTTCCTGCAGCGGCCGCTACTAGTATCCCACCCAGAATAACCAACTTTAT
Line 106: Line 106:
|Amplify the natural rbs of FlhD
|Amplify the natural rbs of FlhD
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O113
+
| style="background: #D4E2EF;" | O113
| FlhDC(nu)-R
| FlhDC(nu)-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGAATAACCAACTTTATTTTTATG
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGAATAACCAACTTTATTTTTATG
Line 113: Line 113:
|Don't amplify the natural rbs of FlhD (only promoter)
|Don't amplify the natural rbs of FlhD (only promoter)
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O114
+
| style="background: #D4E2EF;" | O114
| FliA-PCR1-F
| FliA-PCR1-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCTGATTAACTGAGACTGACGGCAACGC
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCTGATTAACTGAGACTGACGGCAACGC
Line 120: Line 120:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O115
+
| style="background: #D4E2EF;" | O115
| FliA-PCR1-R
| FliA-PCR1-R
| CAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCCGCAGGGTTTTTAATCG
| CAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCCGCAGGGTTTTTAATCG
Line 127: Line 127:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O116
+
| style="background: #D4E2EF;" | O116
| FliA-PCR2-F
| FliA-PCR2-F
| GAAACGGATAATCATGCCGATAACTCATATAACGCAGGGCTG
| GAAACGGATAATCATGCCGATAACTCATATAACGCAGGGCTG
Line 134: Line 134:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O117
+
| style="background: #D4E2EF;" | O117
| FliA-PCR2-R
| FliA-PCR2-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACCATTACACCTTCAGCGGTATAGAGTGAACTCACGATAAA
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACCATTACACCTTCAGCGGTATAGAGTGAACTCACGATAAA
Line 141: Line 141:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O118
+
| style="background: #D4E2EF;" | O118
| FliC-F
| FliC-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCAGCGATGAAATACTTGCCATGCGATT
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCAGCGATGAAATACTTGCCATGCGATT
Line 148: Line 148:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O119
+
| style="background: #D4E2EF;" | O119
| FliC-R
| FliC-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGCGAGAGGCTGTTGGTATTAATGACTT
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGCGAGAGGCTGTTGGTATTAATGACTT
Line 155: Line 155:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O120
+
| style="background: #D4E2EF;" | O120
| FliE-F
| FliE-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGTCGCATTCATCGCGCACCTCGTGGCTG
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGTCGCATTCATCGCGCACCTCGTGGCTG
Line 162: Line 162:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O121
+
| style="background: #D4E2EF;" | O121
| FliE-R
| FliE-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACTGGCTGATAACCCCTTCAATCCCCTGTA
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACTGGCTGATAACCCCTTCAATCCCCTGTA
Line 169: Line 169:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O122
+
| style="background: #D4E2EF;" | O122
| FliF-F
| FliF-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT
Line 176: Line 176:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O123
+
| style="background: #D4E2EF;" | O123
| FliF-R
| FliF-R
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT
Line 183: Line 183:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O124
+
| style="background: #D4E2EF;" | O124
| FliL-F
| FliL-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCGAGAGGCTGTTGGTATTAATGACT
| GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCGAGAGGCTGTTGGTATTAATGACT
Line 190: Line 190:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O125
+
| style="background: #D4E2EF;" | O125
| FliL-R
| FliL-R
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACCAGCGATGAAATACTTGCCATGCGATTT
| GTTTCTTCCTGCAGCGGCCGCTACTAGTACCAGCGATGAAATACTTGCCATGCGATTT
Line 197: Line 197:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O126
+
| style="background: #D4E2EF;" | O126
| Gene-EnvZ-F
| Gene-EnvZ-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGGCGATTGCGCTTCTCGCCAC
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGGCGATTGCGCTTCTCGCCAC
Line 204: Line 204:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O127
+
| style="background: #D4E2EF;" | O127
| Gene-EnvZ-R
| Gene-EnvZ-R
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTACCCTTCTTTTGTCGTGCCCTGCGCC
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTACCCTTCTTTTGTCGTGCCCTGCGCC
Line 211: Line 211:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O128
+
| style="background: #D4E2EF;" | O128
| Gene-FlgM-F
| Gene-FlgM-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTATTGATCGCACTTCGCCTC
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTATTGATCGCACTTCGCCTC
Line 218: Line 218:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O129
+
| style="background: #D4E2EF;" | O129
| Gene-FlgM-R
| Gene-FlgM-R
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGTTACTCTGCAAGTCTTGCTGCGCT
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGTTACTCTGCAAGTCTTGCTGCGCT
Line 225: Line 225:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O130
+
| style="background: #D4E2EF;" | O130
| Gene-FlhC-F
| Gene-FlhC-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTGAAAAAAGCATTGTTCAGG
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTGAAAAAAGCATTGTTCAGG
Line 232: Line 232:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O131
+
| style="background: #D4E2EF;" | O131
| Gene-FlhC-R
| Gene-FlhC-R
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAAACAGCCTGTACTCTCTGTTCATCC
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAAACAGCCTGTACTCTCTGTTCATCC
Line 239: Line 239:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O132
+
| style="background: #D4E2EF;" | O132
| Gene-FlhD-F
| Gene-FlhD-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTGAAAC
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTGAAAC
Line 246: Line 246:
|Don't amplify the natural rbs of FlhD
|Don't amplify the natural rbs of FlhD
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O133
+
| style="background: #D4E2EF;" | O133
| Gene-FlhD-R
| Gene-FlhD-R
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGGCCCTTTTCTTGCGCAGCGCTTCT
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGGCCCTTTTCTTGCGCAGCGCTTCT
Line 253: Line 253:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O134
+
| style="background: #D4E2EF;" | O134
| Gene-FlhDCrbs-F
| Gene-FlhDCrbs-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGACCGCATAAAAATAAAGTTGGTTAT
| GTTTCTTCGAATTCGCGGCCGCTTCTAGACCGCATAAAAATAAAGTTGGTTAT
Line 260: Line 260:
|Amplify the natural rbs of FlhD so that we have FlhD and FlhC with both their natural rbs
|Amplify the natural rbs of FlhD so that we have FlhD and FlhC with both their natural rbs
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O135
+
| style="background: #D4E2EF;" | O135
| Gene-FliA-F
| Gene-FliA-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATTCACTCTATACCGCTGAAG
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATTCACTCTATACCGCTGAAG
Line 267: Line 267:
|Don't Amplify the natural rbs of FliA
|Don't Amplify the natural rbs of FliA
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O136
+
| style="background: #D4E2EF;" | O136
| Gene-FliA-R
| Gene-FliA-R
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATAACTTACCCAGTTTAGTGCGTAAC
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATAACTTACCCAGTTTAGTGCGTAAC
Line 275: Line 275:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O137
+
| style="background: #D4E2EF;" | O137
| Gene-FliArbs-F
| Gene-FliArbs-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTAATCATGCCGATAACTCATATAAC
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTAATCATGCCGATAACTCATATAAC
Line 282: Line 282:
|Amplify the natural rbs of FliA
|Amplify the natural rbs of FliA
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O138
+
| style="background: #D4E2EF;" | O138
| Gene-OmpR-F
| Gene-OmpR-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAAGAGAACTACAAGATTCTGG
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAAGAGAACTACAAGATTCTGG
Line 289: Line 289:
|
|
|- style="background: #cccccc;"
|- style="background: #cccccc;"
-
| style="background: #ccffcc;" | O139
+
| style="background: #D4E2EF;" | O139
| Gene-OmpR-R
| Gene-OmpR-R
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATGCTTTAGAGCCGTCCGGTACAAAG
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATGCTTTAGAGCCGTCCGGTACAAAG

Revision as of 14:38, 30 July 2008

Designed Oligos

Table of Oligos
Number Name Sequence Length Tm(°C) Comments
O100 FlgA-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCATATCTCCTCCGCAGGTATCAAAAT 58
O101 FlgA-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAACAGTATCGCGATGATCGCCACGCTACGT 58
O102 FlgB-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGACAGTATCGCGATGATCGCCACGCTACG 58
O103 FlgB-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGCATATCTCCTCCGCAGGTATCAAAATT 58
O104 FlgK-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCAGATGAAATCGATAAGCGACAAGG 58
O105 FlgK-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAAACGCCGCCTGGGCCGCGTTCAGTCCGCT 58
O106 FlgM-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCGGCGTTGTTGATGCAGATGGGAATA 58
O107 FlgM-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACGAAGTGCGATCAATACTCATGGTTTATT 58
O108 FlhB-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACGTCATATCAGGCGGTCTGATAAGG 58
O109 FlhB-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTTTTGTCGTCGCTCTCGTCAGACACGTC 58
O110 FlhDC-Uri-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGTATGTGCGTGTAGTGACGAGTACAG 58 Don't amplify the both OmpR binding site
O111 FlhDC-Total-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTCATTTTTGCTTGCTAGCGTACGGAAAA 58 Amplify the both OmpR binding site
O112 FlhDC(rbs)-R GTTTCTTCCTGCAGCGGCCGCTACTAGTATCCCACCCAGAATAACCAACTTTAT 54 Amplify the natural rbs of FlhD
O113 FlhDC(nu)-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGAATAACCAACTTTATTTTTATG 54 Don't amplify the natural rbs of FlhD (only promoter)
O114 FliA-PCR1-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCTGATTAACTGAGACTGACGGCAACGC 58
O115 FliA-PCR1-R CAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCCGCAGGGTTTTTAATCG 59
O116 FliA-PCR2-F GAAACGGATAATCATGCCGATAACTCATATAACGCAGGGCTG 42
O117 FliA-PCR2-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACCATTACACCTTCAGCGGTATAGAGTGAACTCACGATAAA 69
O118 FliC-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCAGCGATGAAATACTTGCCATGCGATT 58
O119 FliC-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGCGAGAGGCTGTTGGTATTAATGACTT 58
O120 FliE-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGTCGCATTCATCGCGCACCTCGTGGCTG 58
O121 FliE-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACTGGCTGATAACCCCTTCAATCCCCTGTA 58
O122 FliF-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT 58
O123 FliF-R GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT 58
O124 FliL-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCGAGAGGCTGTTGGTATTAATGACT 58
O125 FliL-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACCAGCGATGAAATACTTGCCATGCGATTT 58
O126 Gene-EnvZ-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGGCGATTGCGCTTCTCGCCAC 53
O127 Gene-EnvZ-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTACCCTTCTTTTGTCGTGCCCTGCGCC 59
O128 Gene-FlgM-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTATTGATCGCACTTCGCCTC 53
O129 Gene-FlgM-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGTTACTCTGCAAGTCTTGCTGCGCT 59
O130 Gene-FlhC-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTGAAAAAAGCATTGTTCAGG 53
O131 Gene-FlhC-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAAACAGCCTGTACTCTCTGTTCATCC 59
O132 Gene-FlhD-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTGAAAC 53 Don't amplify the natural rbs of FlhD
O133 Gene-FlhD-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGGCCCTTTTCTTGCGCAGCGCTTCT 59
O134 Gene-FlhDCrbs-F GTTTCTTCGAATTCGCGGCCGCTTCTAGACCGCATAAAAATAAAGTTGGTTAT 53 Amplify the natural rbs of FlhD so that we have FlhD and FlhC with both their natural rbs
O135 Gene-FliA-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATTCACTCTATACCGCTGAAG 53 Don't Amplify the natural rbs of FliA
O136 Gene-FliA-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATAACTTACCCAGTTTAGTGCGTAAC 59
O137 Gene-FliArbs-F GTTTCTTCGAATTCGCGGCCGCTTCTAGTAATCATGCCGATAACTCATATAAC 53 Amplify the natural rbs of FliA
O138 Gene-OmpR-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAAGAGAACTACAAGATTCTGG 53
O139 Gene-OmpR-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATGCTTTAGAGCCGTCCGGTACAAAG 59



Retrieved from "http://2008.igem.org/Team:Paris/Notebook/Oligo"