Team:University of Sheffield /Project

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(Project Overview)
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====Project Overview====
====Project Overview====
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|The aim is to engineer a biological machine that will sense microbial contamination of drinking water using quorum sensing.
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The aim is to engineer a biological machine that will sense microbial contamination of drinking water using quorum sensing. Quorum sensing systems use sensing proteins in the membrane (histidine-kinases in this case) to detect molecules excreted by the bacteria’s own species. After detection in the membrane, a phosphate signal is passed down pathway, causing a DNA regulatory effect. Each species also has its own corresponding quorum-producing protein. In Vibrio cholerae the sensing protein is CqsS and the quorum-producing protein CqsA, and in Legionella they are LqsS and LqsA.
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The organism would initially be designed to sense a wide range of pathogens – the coliform bacterias, Escherichia coli, Shigella, Campylobacter, Salmonella enteritidis, Helicobacter sp., Vibrio cholera, Mycobacterium avium and the protozoans Giardia lamblia, Cryptosporidium sp., Cyclospora sp. and Toxoplasma sp. These cause a range of serious diseases such as dysentery, typhoid, cholera and many gastrointestinal infections.
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====History of development====
====History of development====

Revision as of 16:43, 31 July 2008

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Overall project

Project Overview

The aim is to engineer a biological machine that will sense microbial contamination of drinking water using quorum sensing. Quorum sensing systems use sensing proteins in the membrane (histidine-kinases in this case) to detect molecules excreted by the bacteria’s own species. After detection in the membrane, a phosphate signal is passed down pathway, causing a DNA regulatory effect. Each species also has its own corresponding quorum-producing protein. In Vibrio cholerae the sensing protein is CqsS and the quorum-producing protein CqsA, and in Legionella they are LqsS and LqsA.

History of development




Project Details

Parts submitted to the Registry



Modelling