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EPF-Lausanne/31 July 2008
From 2008.igem.org
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The 2nd step went well. All 3 worked. We will now be able to use these DNA strands to make our transcription fractors and test on the chips to see where they actualy bind. <br> | The 2nd step went well. All 3 worked. We will now be able to use these DNA strands to make our transcription fractors and test on the chips to see where they actualy bind. <br> | ||
We have to determine the consensus binding region for each to have a starting point. </p> | We have to determine the consensus binding region for each to have a starting point. </p> | ||
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| + | ===Cell culture === | ||
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| + | We did again a culture from the igem stock the headquarters send us. We cultured the following biobricks : I14033, I1466, R0071, B0034, B0015, P0140, R0040, E1010, F1610. | ||
Revision as of 13:59, 31 July 2008
Ligation of parts
We digested a few parts yesterday and we ligated them this morning
2 Step PCR
The 2nd step went well. All 3 worked. We will now be able to use these DNA strands to make our transcription fractors and test on the chips to see where they actualy bind.
We have to determine the consensus binding region for each to have a starting point.
Cell culture
We did again a culture from the igem stock the headquarters send us. We cultured the following biobricks : I14033, I1466, R0071, B0034, B0015, P0140, R0040, E1010, F1610.
