Team:Valencia/Project

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Revision as of 16:50, 1 August 2008

Heat is vital for life. Many living forms are unable to maintain his temperature in an adequate range; while others keep it constant using several biochemical mechanisms. We thought it would be really interesting to implement some of these biological tools in an organism that we could easily control.

Using yeast as our model, we aim to be able to express a common mammal protein, thermogenine, to produce heat. This process would be really useful in many industrial applications and even daily routine actions.

We are using Saccaromyces cerevisiae strains handed over by [http://www.cbm.uam.es/mitolab/fichapersonal.aspx?idpersona=6|Dr. Eduardo Rial]. Besides, we have built our own calorimeters so as to record the temperature differences.

In a later stage, we expect to control this temperature differences to optimize the possible applications. Consequently, we will implement a regulatory system for the thermogenine gene expression.

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-==Project Overall==
-===Stage 1===
-Uncoupling protein from the brown adipose tissue (UCP1) is a transporter that catalyzes a regulated discharged of the mitochondrial proton gradient. We aim to express this transmembrane protein in ''Saccaromyces Cerevisiae'' so as to be able to detect a temperature difference from the control.
-We are using four different strains: one expresing UCP1, two mutants that overexpress UCP1, and a control one. All of this stains have been handed over by CSIC researcher Dr. Eduardo Rial.
+Heat is vital for life. Many living forms are unable to maintain his temperature in an adequate range; while others keep it constant using several biochemical mechanisms. We thought it would be really interesting to implement some of these biological tools in an organism that we could easily control.
-To record temperature we are using calorimeters which consist on insulated flasks with attached thermocouples.
+Using yeast as our model, we aim to be able to express a common mammal protein, thermogenine, to produce heat. This process would be really useful in many industrial applications and even daily routine actions.
-===Stage 2===
+We are using ''Saccaromyces cerevisiae'' strains handed over by [http://www.cbm.uam.es/mitolab/fichapersonal.aspx?idpersona=6|Dr. Eduardo Rial]. Besides, we have built our own calorimeters so as to record the temperature differences.
-In a later stage, we expect to control this temperature differences to optimize the possible applications. Consequently, we will implement a regulatory system of UCP1 gene expression.
+In a later stage, we expect to control this temperature differences to optimize the possible applications. Consequently, we will implement a regulatory system for the thermogenine gene expression.
-===Aplications===
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