User:University of Washington/31 July 2008

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Revision as of 23:50, 31 July 2008

LuxR from AraC and TetR

- minipreped BioBrick E0240

- ran gel for E0240: All 5 lanes had plasmids with same length.

- Transformed BBa_P1010 (contain death gene, cut from binder) into DB and XL1-Blue, then inoculated on Amp LB plate.

Cure strain MG1655Z1

- minipreped Elowitz's plasmids

- ran gel for Elowitz's plasmid: Lane 2-5(colonies selected from plate of Glycerol Stock E.Coli) contained plasmids with same length. Lane 1(colony selected from original plate from Elowitz's lab) showed two bands, non of which matched with the rest of the lanes. Will further investigate the reasons tomorrow by restriction digestion of plasmid and compare.

- Selected 8 colonies of MG1655Z1 from Tsy plate and streaked out in new Tsy plate.

Back to Team:University_of_Washington/Notebook#Notebook

@@ Line 1: / Line 1: @@
 ==LuxR from AraC and TetR==
-- minipreped Elowitz's plasmids and BioBrick E0240
+- minipreped BioBrick E0240
-- ran gel
+- ran gel for E0240: All 5 lanes had plasmids with same length.
-*E0240: All 5 lanes had plasmids with same length.
-*Elowitz's: Lane 2-5(colonies selected from plate of Glycerol Stock E.Coli) contained plasmids with same length. Lane 1(colony selected from original plate from Elowitz's lab) showed two bands, non of which matched with the rest of the lanes. Will further investigate the reasons tomorrow by restriction digestion of plasmid and compare.
-- Selected 8 colonies of MG1655Z1 from Tsy plate and streaked out in new Tsy plate.
+- Transformed BBa_P1010 (contain death gene, cut from binder) into DB and XL1-Blue, then inoculated on Amp LB plate.
-- Transformed BBa_P1010 (cut from binder) into DB and XL1-Blue, then inoculated on Amp LB plate.
+==Cure strain MG1655Z1==
+- minipreped Elowitz's plasmids
+- ran gel for Elowitz's plasmid: Lane 2-5(colonies selected from plate of Glycerol Stock E.Coli) contained plasmids with same length. Lane 1(colony selected from original plate from Elowitz's lab) showed two bands, non of which matched with the rest of the lanes. Will further investigate the reasons tomorrow by restriction digestion of plasmid and compare.
+- Selected 8 colonies of MG1655Z1 from Tsy plate and streaked out in new Tsy plate.
 ----
 Back to [[Team:University_of_Washington/Notebook#Notebook]]