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Team:UNIPV-Pavia/Notebook/Week1
From 2008.igem.org
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==Week 1: 05/19/08 - May 05/23/08== | ==Week 1: 05/19/08 - May 05/23/08== | ||
| + | |||
| + | '''05/19/08''' | ||
| + | Let’s start our IGEM 2008 experience! | ||
| + | At first, we broke the punch tool…:) | ||
| + | |||
| + | We used a scalpel to cut and resuspend the following 22 paper spots: | ||
| + | |||
| + | BBa_I14032 BBa_R0079 BBa_R0062 BBa_R0040 | ||
| + | BBa_R0082 BBa_R0051 BBa_J23100 BBa_C0161 | ||
| + | BBa_C0062 BBa_C0078 BBa_C0179 BBa_C0051 | ||
| + | BBa_C0012 BBa_C0040 BBa_I15010 BBa_I15008 | ||
| + | BBa_I15009 BBa_E0040 BBa_E1010 BBa_B0030 | ||
| + | BBa_B1006 BBa_E0240 | ||
| + | |||
| + | We used tweezers to put the cut paper into tubes containing 10 μl of warmed TE buffer. | ||
| + | |||
| + | We transformed 60 µl of TOP10 E. coli with 4 µl of DNA in TE for all 22 parts, plated transformed bacteria and incubated overnight at 37°C. | ||
| + | |||
| + | We used LB medium previously prepared, with the suitable antibiotic added. | ||
Revision as of 17:32, 27 May 2008
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|
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Notebook
| Week 1 | Week 2 |
|---|
Week 1: 05/19/08 - May 05/23/08
05/19/08 Let’s start our IGEM 2008 experience! At first, we broke the punch tool…:)
We used a scalpel to cut and resuspend the following 22 paper spots:
BBa_I14032 BBa_R0079 BBa_R0062 BBa_R0040 BBa_R0082 BBa_R0051 BBa_J23100 BBa_C0161 BBa_C0062 BBa_C0078 BBa_C0179 BBa_C0051 BBa_C0012 BBa_C0040 BBa_I15010 BBa_I15008 BBa_I15009 BBa_E0040 BBa_E1010 BBa_B0030 BBa_B1006 BBa_E0240
We used tweezers to put the cut paper into tubes containing 10 μl of warmed TE buffer.
We transformed 60 µl of TOP10 E. coli with 4 µl of DNA in TE for all 22 parts, plated transformed bacteria and incubated overnight at 37°C.
We used LB medium previously prepared, with the suitable antibiotic added.
