Alberta NINT/14 May 2008

From 2008.igem.org

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(New page: lab work (SD): Isolated plasmid DNA from culture tubes using QIAprep miniprep standard protocol. Digested DNA with EcoRI + PstI. Incubated at 37 C for 2 hrs. Run on 2% agarose gel. Exp...)
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lab work (SD): Isolated plasmid DNA from culture tubes using QIAprep miniprep standard protocol. Digested DNA with EcoRI + PstI.  Incubated at 37 C for 2 hrs.  Run on 2% agarose gel.  Expected fragment lengths: R0010 200 bp, R0011 55 bp, E0433 533 bp, pSB1A2 ~2 kbp.
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lab work (SD): Isolated plasmid DNA from culture tubes using QIAprep miniprep standard protocol.  
 +
              Digested DNA with EcoRI + PstI.   
 +
              Incubated at 37 C for 2 hrs.  Run on 2% agarose gel.   
 +
              Expected fragment lengths: R0010 200 bp, R0011 55 bp, E0433 533 bp, pSB1A2 ~2 kbp.

Revision as of 02:28, 6 June 2008

lab work (SD): Isolated plasmid DNA from culture tubes using QIAprep miniprep standard protocol. 

              Digested DNA with EcoRI + PstI.  
              Incubated at 37 C for 2 hrs.  Run on 2% agarose gel.  
              Expected fragment lengths: R0010 200 bp, R0011 55 bp, E0433 533 bp, pSB1A2 ~2 kbp.
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