Team:Paris/August 1

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== MiniPreps ==
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* Use of Promega's protocol on all the clones cultivated on the 24th.
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* Preparation of 50µl of minipreps in simple.
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== List of Minipreps ==
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{| border="1"
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|align="center"|'''Name'''
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|align="center"|'''Biobrick'''
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|align="center"|'''Description'''
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|-
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|align="center"|MP100
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|align="center"|[http://partsregistry.org/Part:BBa_B0034 B0034]
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|align="center"|Strongest RBS (Efficiency = 1)
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|-
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== Transformations ==
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=== Protocol ===
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use of TOP10 Chemically competent cells
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* Defroze competent cells on ice during 5'
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* Add 5µl of DNA Ligation in 50µL of competent bacterias (or 1µL for the positive control puc19)
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* Incubate 30' on ice
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* Heat-shock the cells during 30" at 42°C without shaking
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* Put 2' on ice
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* Add 250µL of pre-warmed SOC medium (4°C)
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* Incubate 1h at 37°C under shaking (225rpm)
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* Spin at 5.000rpm during 30"
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* Remove 150µL of supernatant
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* Resuspent the pellet in the 150µL left
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* Spread on adequated plates
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* Incubate O/N at 37°C
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=== List of transformation ===

Revision as of 11:52, 4 August 2008

minipreps

souche stockage


Contents

MiniPreps

  • Use of Promega's protocol on all the clones cultivated on the 24th.
  • Preparation of 50µl of minipreps in simple.


List of Minipreps

Transformations

Protocol

use of TOP10 Chemically competent cells

  • Defroze competent cells on ice during 5'
  • Add 5µl of DNA Ligation in 50µL of competent bacterias (or 1µL for the positive control puc19)
  • Incubate 30' on ice
  • Heat-shock the cells during 30" at 42°C without shaking
  • Put 2' on ice
  • Add 250µL of pre-warmed SOC medium (4°C)
  • Incubate 1h at 37°C under shaking (225rpm)
  • Spin at 5.000rpm during 30"
  • Remove 150µL of supernatant
  • Resuspent the pellet in the 150µL left
  • Spread on adequated plates
  • Incubate O/N at 37°C


List of transformation

Name Biobrick Description
MP100 [http://partsregistry.org/Part:BBa_B0034 B0034] Strongest RBS (Efficiency = 1)
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