Minnesota/4 August 2008

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Revision as of 20:16, 4 August 2008

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1. Send in products _______ for sequencing

2. Meeting with Yiannis: (1) Make sure powerpoint does not exceed 30 minutes, send it to Yiannis on Wednesday for him to review. (2) Write paper with materials and methods that can point to specific papers that we followed instead of writing out entire procedures. (3) Have iGEM requirements all finished before week ends.

3. Ligations: Ligate the digested products from 08-01-08. Follow the table below:

Parts	10x Buffer	H20	prev. lig. + BV DNA	Insert DNA	T4 DNA Ligase	Total
BV + Tet/p22 + RFP + Term	4.0uL	4.0uL	5.0uL	23.0uL	4.0uL	40.0uL
BV + Tet pro + LAMBDAcI + Term	4.0uL	4.0uL	5.0uL	23.0uL	4.0uL	40.0uL
BV + Lac/LAMBDA + GFP 1	4.0uL	4.0uL	5.0uL	23.0uL	4.0uL	40.0uL
BV + Lac/LAMBDA + GFP 2	4.0uL	4.0uL	5.0uL	23.0uL	4.0uL	40.0uL

@@ Line 11: / Line 11: @@
 |-
 |'''2. Meeting with Yiannis:''' (1) Make sure powerpoint does not exceed 30 minutes, send it to Yiannis on Wednesday for him to review. (2) Write paper with materials and methods that can point to specific papers that we followed instead of writing out entire procedures. (3) Have iGEM requirements all finished before week ends.
+|-
+|'''3. Ligations:''' Ligate the digested products from 08-01-08. Follow the table below:
+{|border="1" align="left"
+|-
+!| Parts !! 10x Buffer!! H20 !! prev. lig. + BV DNA !! Insert DNA !! T4 DNA Ligase !! Total
+|-
+| BV + Tet/p22 + RFP + Term || 4.0uL || 4.0uL || 5.0uL || 23.0uL || 4.0uL || 40.0uL
+|-
+| BV + Tet pro + LAMBDAcI + Term || 4.0uL || 4.0uL || 5.0uL || 23.0uL || 4.0uL || 40.0uL
+|-
+| BV + Lac/LAMBDA + GFP 1 || 4.0uL || 4.0uL || 5.0uL || 23.0uL || 4.0uL || 40.0uL
+|-
+| BV + Lac/LAMBDA + GFP 2 || 4.0uL || 4.0uL || 5.0uL || 23.0uL || 4.0uL || 40.0uL
+|}