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Team:KULeuven/6 August 2008
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Check possibility of hybrid promotor to connect memory and timer (LuxI). This is to replace the antisense LuxI. Promotor would look something like this: <br> | Check possibility of hybrid promotor to connect memory and timer (LuxI). This is to replace the antisense LuxI. Promotor would look something like this: <br> | ||
| - | Operator<sub>HSL_LuxR</sub> '''---''' -35 box '''---''' Operator<sub>CII P22</sub> '''---''' -10 box | + | Operator<sub>HSL_LuxR</sub> '''---''' -35 box '''---''' Operator<sub>CII P22</sub> '''---''' -10 box |
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| + | New modeling of the antisense LuxI and its target LuxI mRNA shows that this leaky repression is not a problem for the system. Still looking at the composite promoter though. | ||
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| + | '''Literature''' | ||
| + | [http://jb.asm.org/cgi/content/full/190/13/4392?view=long&pmid=18083819 Lux regulatory region (pLux)] | ||
== Modeling == | == Modeling == | ||
Revision as of 13:15, 6 August 2008
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Contents |
Lab Work
Wet Lab
Dry Lab
Check possibility of hybrid promotor to connect memory and timer (LuxI). This is to replace the antisense LuxI. Promotor would look something like this:
OperatorHSL_LuxR --- -35 box --- OperatorCII P22 --- -10 box
New modeling of the antisense LuxI and its target LuxI mRNA shows that this leaky repression is not a problem for the system. Still looking at the composite promoter though.
Literature
[http://jb.asm.org/cgi/content/full/190/13/4392?view=long&pmid=18083819 Lux regulatory region (pLux)]
Modeling
Wiki
Dr. Coli is everywhere, even at the URL.
Remarks
Discovery of the day. Two people beat us to the punch: [http://www.diagnosticinformationsystem.com/bios.html 1] and [http://www.sscofcny.com/doctors/coli.htm 2]
