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Alberta NINT/30 July 2008
From 2008.igem.org
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(New page: < Back to notebook < Previous entry lab work (SD): Extracted DNA from gel using QIAquick gel extraction prot...) |
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Quantified DNA and ligated K102031 /E+P and K102032 /E+P together. | Quantified DNA and ligated K102031 /E+P and K102032 /E+P together. | ||
Transformed XL1-B cells with the ligation product. | Transformed XL1-B cells with the ligation product. | ||
| + | |||
| + | lab work (JD): | ||
| + | Ligation of K102011 with TAIn_2, TAIn_3, TAIn_6 and TAIn_7 | ||
| + | Transformed XL1-B cells with the ligations | ||
| + | Plated on LB+kan35 plates | ||
| + | Incubated at 37C overnight | ||
[[Alberta_NINT/31_July_2008 | Next entry >]] | [[Alberta_NINT/31_July_2008 | Next entry >]] | ||
Revision as of 18:14, 6 August 2008
lab work (SD):
Extracted DNA from gel using QIAquick gel extraction protocol.
Quantified DNA and ligated K102031 /E+P and K102032 /E+P together.
Transformed XL1-B cells with the ligation product.
lab work (JD):
Ligation of K102011 with TAIn_2, TAIn_3, TAIn_6 and TAIn_7
Transformed XL1-B cells with the ligations
Plated on LB+kan35 plates
Incubated at 37C overnight
