Alberta NINT/6 August 2008
From 2008.igem.org
(Difference between revisions)
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From plates of yesterday's ligations there were NO colonies | From plates of yesterday's ligations there were NO colonies | ||
Replate cells on bottom of tubes on LB+kan35 again | Replate cells on bottom of tubes on LB+kan35 again | ||
- | Carried out calculations to better determine appropriate dilutions and protocol for annealing, digestion and ligation of newly created oligos. | + | Carried out calculations to better determine appropriate dilutions and protocol for annealing, |
+ | digestion and ligation of newly created oligos. | ||
[[Alberta_NINT/7_August_2008 | Next entry >]] | [[Alberta_NINT/7_August_2008 | Next entry >]] |
Revision as of 18:17, 11 August 2008
lab work (JD):
From plates of yesterday's ligations there were NO colonies Replate cells on bottom of tubes on LB+kan35 again Carried out calculations to better determine appropriate dilutions and protocol for annealing, digestion and ligation of newly created oligos.