Alberta NINT/6 August 2008

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               From plates of yesterday's ligations there were NO colonies
               From plates of yesterday's ligations there were NO colonies
               Replate cells on bottom of tubes on LB+kan35 again
               Replate cells on bottom of tubes on LB+kan35 again
-
               Carried out calculations to better determine appropriate dilutions and protocol for annealing, digestion and ligation of newly created oligos.
+
               Carried out calculations to better determine appropriate dilutions and protocol for annealing,  
 +
              digestion and ligation of newly created oligos.
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Revision as of 18:17, 11 August 2008

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lab work (JD):

             From plates of yesterday's ligations there were NO colonies
             Replate cells on bottom of tubes on LB+kan35 again
             Carried out calculations to better determine appropriate dilutions and protocol for annealing, 
             digestion and ligation of newly created oligos.

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